RNA interference inhibition of factor -1 gene expression in differentiation of esophageal squamous cell carcinoma proliferation and apoptosis.doc
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RNA interference inhibition of factor -1 gene expression in differentiation of esophageal squamous cell carcinoma proliferation and apoptosis
RNA interference inhibition of factor -1 gene expression in differentiation of esophageal squamous cell carcinoma proliferation and apoptosis
Of: have reached Wu, Fang Mingxia, Liu Yu Rui
[Abstract] Objective To study the differentiation inhibitory factor -1 for the siRNA (si-Id-1) in human esophageal squamous cell carcinoma (ESCC) cell proliferation and the possible mechanisms of apoptosis. Methods of cationic liposome-mediated si- Id-1 transfected ESCC TE-1 cell line, cells were observed under inverted fluorescence microscope, morphological changes and calculate transfection efficiency, RT-PCR detection of mRNA expression of Id-1 level; detected by Western blot the expression of Id-1 protein levels ; cell proliferation assay transfected TE-1 cell proliferation changes; flow cytometry to detect the cell cycle and apoptotic index. Results si-Id-1 before and after transfection of TE-1 cell line Id-1 The mRNA and protein levels were significantly different (P lt;0.05); transfected TE-1 cells than their parental cells decreased proliferation, apoptosis levels increased (P lt;0.05); transfected TE-1 cell cycle arrest in G0/G1 phase, G1 phase cells increased, S phase cells decreased (P lt;0.05). Conclusion liposome si-Id-1 transfected into the TE-1 ESCC cells is feasible; si -Id-1 can effectively silence the TE-1 cells the expression of Id-1, thereby inhibiting the proliferation of ESCC cells.
[Keywords:] inhibitory factor, immune; carcinoma, squamous cell; esophageal cancer; RNA, small interfering; apoptosis; cell proliferation
In China, esophageal cancer has a high incidence and high mortality, with surgery, radiation therapy or chemotherapy combined with comprehensive treatment can improve survival rate, but still 90% of patients died of metastasis and local recurrence [1] Therefore, an effective search for new treatments or strategies of esophageal cancer is imminent. differentiation inhibitory factor (inhibitor of DNA binding or differentiation, Id-1) in
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