RP-HPLC on the bone content of ferulic acid capsules.doc

 PAGE \* MERGEFORMAT 8 RP-HPLC on the bone content of ferulic acid capsules [Abstract] Objective To establish a bone capsule for determining the content of ferulic acid. Methods HPLC, column: Hypersil C18 (4.6 mm × 250 mm, 5 μm), mobile phase was methanol -1% ice acetic acid (33:67), detection wavelength was 320 nm, flow rate 1.0 mL / min. Results of ferulic acid in the range of 0.12 ~ 1.2 μg linear relationship (r = 0.999 9), the average recovery was 99.6% , RSD = 1.8%. Conclusion The method is simple, accurate, and can be used to control the quality of bone capsules. Keywords:] bone capsule, ferulic acid, HPLC, determination Abstract: Objective To establish a method for determination of Ferulic acid in Jiegu Capsule. Method The samples were determined by RP-HPLC on a Hypersil C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol and 1% acetic acid (33:67). The detection wavelength was at 320 nm and the flow rate was 1.0 mL / min. Results The calibration curves of Ferulic acid was linear in the range of 6 ~ 60 μg / mL (r = 0.999 9) . The average recovery was 99.6% and RSD was 1.8%. Conclusion The method is simple and accurate, which can be used for the quality control of Jiegu Capsule. Keywords:: Jiegu Capsule, Ferulic acid; HPLC, content determination Capsule is in the bone pieces bone [“Chinese Ministry of Health, prescription drug standard preparation” (Volume 2) WS3-B-0402-90] based on the development of new agents, by the fourth copper, Chuan Xiong, Radix system , angelica, frankincense (vinegar Sunburn), myrrh (vinegar Sunburn), native copper (calcined) composed of 7 herbs, with stasis, promoting blood circulation, analgesic efficacy, clinical for traumatic injury, tendon injury fractures, bleeding sore . ferulic acid-based products one of the active ingredient, and higher levels. This test uses high performance liquid chromatography in the bone capsule of the determination of ferulic acid, to control the quality of t