Salbutamol on rat dendritic cells in the regulation.docVIP

 PAGE \* MERGEFORMAT 15 Salbutamol on rat dendritic cells in the regulation Study: Lake Wang, Zhang Hui, Huang Lili, Shao Qixiang [Abstract] Objective: To study the 2 agonist salbutamol regulation of dendritic cells (dendritic cells, DCs) immune function. METHODS: The recombinant rat granulocyte - colony stimulating factor (recombinant rat granulocyte monocytes colony stimulating factor, rrGM CSF and recombinant rat interleukin-4 (recombinant rat interleukin 4, rrIL 4 from the SD rat bone marrow cells induced from immature DCs, and then with LPS induced maturation. DCs, respectively, and combined in the 2 agonist salbutamol and its added resistance off oxygen dose D amine, light microscopy and confocal microscopy of salbutamol on the morphology of DCs by flow cytometry analysis of DCs surface markers changes, ultimate DCs by mixed lymphocyte reaction for the detection function. Results: inverted Under a microscope, cell morphology was found between the experimental and control groups were significantly different, flow cytometry analysis showed that: Salbutamol increased the relative specific markers OX62 DCs in rats the expression of costimulatory molecules reduced CD80, CD86 and MHC molecules, but the signs CD11c myeloid no significant effect on oxygen amine D reduced CD11c, MHC , CD80 and CD86 expression, but no significant effect on the expression of OX62, salbutamol combined with the small amine oxide can significantly lower CD80 and CD86, MHC , CD11c expression, increased OX62 expression, mixed lymphocyte reaction showed that salbutamol can inhibit DCs ability to stimulate T cell proliferation, small oxygen amines also inhibit the proliferation of DCs to stimulate T cells in the ability of salbutamol combined D amine oxide DCs also inhibit the ability to stimulate T cell proliferation, neither Dante oxygen was blocked amine the effects of salbutamol, no significant synergy. Conclusion: 2 agonist salbutamol DCs by decreasing the surface MHC and cost