Salmonella typhi genome DNA chip gene expression analysis and optimization of the establishment.doc

 PAGE \* MERGEFORMAT 24 Salmonella typhi genome DNA chip gene expression analysis and optimization of the establishment Study: Health Xiu, Huang Xinxiang, Chee Soon Juan High, Zhang Fang, Xu of the River [Abstract] Objective: To establish and optimize the Salmonella typhi-based whole genome DNA chip gene expression analysis. Method: extract of wild z66 positive Salmonella typhi isolates total RNA, the nucleotide random primers 6,9 (N6, N9 and (or genome specific primers (GDP reverse transcription synthesis of cDNA, using fluorescent Cy5 or Cy3 direct incorporation of the tags, and include Salmonella typhi 4201 protein coding genes of the Salmonella typhi genome microarray, with the chip scanner scans obtained after expression profile analysis results, after the data were analyzed after standardization. Results: N9 + GDP mixed primers, direct incorporation tags, both to obtain better results mark, but also save materials costs, while reducing red tape caused by the steps unnecessary mistakes. Conclusion: The genome of Salmonella typhi-based DNA chip analysis of gene expression profiling technology for regulating gene expression in bacteria and functional genomics provide a platform. [Keywords:] Salmonella typhi, genomic, DNA chip, gene expression profiling [Abstract] Objective: To develop and optimize the Salmonella enterica serovar Typhi genomic DNA microarray based gene expression profiling. Methods: Total RNA of the z66 positive wild strains of Salmonella enterica serovar Thypi was extracted and reverse transcribed to cDNA with random hexa oligonucleotide primers ( N6), nona oligonucleotide primers (N9) and genome directed primers (GDP). During reverse transcription, cDNA was labeled with Cy3 dye or Cy5 dye directly. The labeled probes were purified and hybridized to a genomic DNA microarrays containing 4201 ORF of S. enterica serovar Typhi. The images were obtained by a laser scanner with two channels and the digital data were analyzed by the Acu