Salmonella typhi genome DNA chip gene expression analysis and optimization of the establishment.docVIP

 PAGE \* MERGEFORMAT 24 Salmonella typhi genome DNA chip gene expression analysis and optimization of the establishment Study: Health Xiu, Huang Xinxiang, Chee Soon Juan High, Zhang Fang, Xu of the River [Abstract] Objective: To establish and optimize the Salmonella typhi-based whole genome DNA chip gene expression analysis. Method: extract of wild z66 positive Salmonella typhi isolates total RNA, the nucleotide random primers 6,9 (N6, N9 and (or genome specific primers (GDP reverse transcription synthesis of cDNA, using fluorescent Cy5 or Cy3 direct incorporation of the tags, and i