Salvia miltiorrhiza determination of tanshinone Ⅱ A Improvement.docVIP

 PAGE \* MERGEFORMAT 6 Salvia miltiorrhiza determination of tanshinone Ⅱ A Improvement Of: Jinwei Rong, Sun Qiang, single Gang, Wang Zhifen [Abstract] Objective of Salvia miltiorrhiza Determination of tanshinone Ⅱ A (USP method and modified method) comparative study analysis to determine the feasibility and advantages of the improved method. Methods in the original high performance liquid chromatography (HPLC) method based on the adopted relationship between changes in the ratio of mobile phase, the original determination to make improvements Pharmacopoeia and USP measured results were compared was determined. Results ① measured by the improved method, chromatographic peak shape is good, tanshinone Ⅱ A separate peak and other peaks degrees and the tailing factor to meet the requirements; ② the same batch of tanshinone Ⅱ A Salvia assay results show that the two methods was no significant difference (Pgt; 0.05); the same time, the determination of ways to improve the measurement time is shortened, saving the mobile phase . Conclusion The improved method of reproducible results are accurate and reliable, saving time and mobile phase of testing, reduce test cost, is recommended as an alternative. [Keywords:] compound Danshen tablets; tanshinone Ⅱ A; determination; Improvement Salvia is widely used in clinical blood stasis and pain, promoting blood circulation, a pure heart Chufan medicine, ““ China Pharmacopoeiagt; “-2005 edition of the Department Ⅰ high performance liquid chromatography tanshinone Ⅱ A content. Found in the actual mining Pharmacopoeia testing work methods of methanol - water (73:27) mobile phase, flow rate 1 ml · min-1, the tanshinone Ⅱ A 21 min retention time about the peak time later, the mobile phase and the detection time to waste. To this end, we tanshinone Ⅱ A on Salvia Determination of mobile phase was improved. An instrument and reagent Agilent 1200 HPLC, Agilent DAD detector, Agilent Chemstation workstation (Agilent Techn