Solid phase extraction - liquid chromatography - tandem mass spectrometry determination of fluoroquinolones in soil tetracycline and sulfonamide antibiotics_0.docVIP

 PAGE \* MERGEFORMAT 17 Solid phase extraction - liquid chromatography - tandem mass spectrometry determination of fluoroquinolones in soil tetracycline and sulfonamide antibiotics Study: Hu Wei, Sha Li-Li Ma Jian Guo Changsheng Zhu Xingwang Ruanyue Fei Wang Yuqiu [Abstract] solid phase extraction - liquid chromatography - tandem mass spectrometry method for simultaneous detection of soil fluoroquinolones, tetracyclines and sulfonamides analysis of 18 kinds of antibiotics. Soil sample containing 50% acetonitrile by phosphate buffer ( pH = 3) extraction, the SAX-HLB column purification of enriched small series, the HPLC / MS / MS multiple reaction monitoring mode for qualitative and quantitative analysis. concentration of 200 and 50 μg / kg, the soil in the fluoroquinolone class, tetracycline, sulfonamides The recoveries were 67.2% ~ 89.0%, 62.2% ~ 85.4% and 55.8% ~ 97.4%, and the relative standard deviation of 1.1% to 17.2%. to 3 times the signal to noise ratio estimate the fluoroquinolones, tetracyclines, sulfonamides of the detection limits were 3.4 ~ 8.9 μg / kg, 0.56 ~ 0.91 μg / kg and 0.07 ~ 1.85 μg / kg. application of this method detected 6 different types of soil samples, the results show that , detected in sewage irrigated soil antibiotics, the concentration of 1.72 ~ 119.6 μg / kg. [Keywords:] fluoroquinolones, tetracycline, sulfonamides, solid phase extraction, high performance liquid chromatography tandem mass spectrometry, soil Abstract An analytical method was developed for the simultaneous extraction and determination of eighteen fluoroquinolones (FQs), tetracyclines (TCs) and sulfonamides (SAs) antibiotics from soils using solid phase extraction and liquid chromatography tandem mass spectrometry.Soil sample was firstly extracted by phosphate buffer at pH 3 in combination with 50% of organic modifier acetonitrile, then purified and concentrated by SAX and HLB column.Qualitative and quantitative analysis were carried out for the analyt