Study of platelet storage lesion.docVIP

 PAGE \* MERGEFORMAT 16 Study of platelet storage lesion Keywords:: platelet glycoprotein storage ultrastructure Platelet storage lesion (ThePlateletStorageLesion, PSL) is from the blood collection, preparation, storage of platelets for the patient to bring this process, platelets due by various physical and chemical factors, morphology and function of damaged. In recent years, clinical increasing demand for platelets, platelet preparation and storage conditions have gradually improved, but the presence of PSL has seriously affected the transmission of platelet recovery rate and life expectancy after the body, weakening the clinical results, even lead to transfusion. Therefore, the mechanism of the PSL of research and preventive measures of exploration into the field of transfusion medicine is an important issue. To the writer’s research in recent years and progress on the PSL are summarized below. A platelet ultrastructural changes 1.1 Morphology Unactivated platelets was smooth two-sided convex discoidal body, a diameter of about 3 ~ 4μ m. Prepared according to standard blood bank fresh platelets, form a minor change, there spinous process, pseudo-feet and weak aggregation. These changes by 37 ° C after 30min incubation reversed [1]. During storage due to activation or metabolic failure leading to impaired barrier function of platelet membrane, causing swelling and even dissolution of platelets. 22 ° C Zhenyao preservation 24h, about 3% to 5% of the platelets dissolved or balloon-like change; save 5d, to 5% ~ 10%; if stored improperly, damaged platelets up to 95% [2]. 1.2 storage of grain α -particles in control of platelets in blood coagulation, inflammation, the repair process functions. Save 3d, α -particle electron density decreased, some of α -particles fuse to form large mixed particles; save 5d, using platelet-rich plasma prepared by platelet (PRP-PC) in the α -particles significantly reduced, but with the tunica albuginea Preparation of plate