TGF-β1 gene modified stem cells induced differentiation into cartilage Study.docVIP

 PAGE \* MERGEFORMAT 18 TGF-β1 gene modified stem cells induced differentiation into cartilage Study Of: WANG Hong-lin, Min Fan red, Wang Yanfeng, Xie Yong, Zhang Jingang, Luan Hong Jia, Hou Xudong [Abstract] [Objective] This study was selected on the synthesis of cartilage ECM can promote the role of TGF-β1 transfection of stem cells (ADSCs, observation of gene after transfection and stable expression of cartilage extracellular matrix (ECM) of the two important components: Ⅱ collagen (type Ⅱ collage, Col Ⅱ) and aggrecan synthesis, in order to build new tissue-engineered cartilage to provide experimental basis. [Method] TGF-β1 gene transfer stem cells and positive cell clone screening; RT -PCR, TGF-β1, fibronectin (fibronectin, FN), Col Ⅱ, Aggrecan expression, Western-blot detection of TGF-β1. [results] RT-PCR results showed that: the experimental group (TGF-β1 stable transfection group TGF-β1, FN, Col Ⅱ, Aggrecan expression compared with empty transfected group and control group was significantly increased (P lt;0. 01, Western blot detection of the experimental group (TGF-β1 stable transfected TGF-β1 expression than the empty transfected group and control group was significantly increased (P lt;0. 01. [Conclusion] The successful isolation and culture of adipose stem cells to liposome-mediated TGF-β1 gene will be successfully transfected ADSCs, after transfection into cartilage differentiation specific increase in extracellular matrix, indicating that TGF-β1 gene transfection can promote the differentiation of ADSCs into chondrocytes, cartilage tissue engineering so as to provide new ideas and methods. [Keywords:] transforming growth factor β1 (TGF-β1, adipose stem cells (ADSCs, gene transfection, RT-PCR, Western-blot Abstract: [Objective] To induce TGF-β1 gene which can increase ECM synthesis into adipose tissue derived stem cells (ADSCs by employing gene transfer techniques and observe whether TGF-β1 gene could expresse continuously and whether ty