Tissue culture method in the application of preservation of articular cartilage.docVIP

 PAGE \* MERGEFORMAT 20 Tissue culture method in the application of preservation of articular cartilage Authors: Song Hongjiang, Qi Jian-Hong, Yanhua Xuan, Bi Hua Abstract [Objective] To investigate the three kinds of preservation methods on articular cartilage cell activity in the different effects to find a good effect of cartilage preservation. [Methods] harvesting adult pig cartilage, is made of about 4.5 mm × 5 mm size of the cylindrical bone cartilage. Using tissue culture method, the slow cooling gradient freezing method, the traditional slow-freezing method of cooling a continuous cartilage save processing, observe and compare the saved cartilage cell activity changes. [Results] save 8 weeks, using the traditional freezing method of the articular cartilage cell viability was less than 50%, a large number of loss of cartilage matrix components; using slow-freezing temperature gradient of cell survival rate of 66%, while the use of tissue-culture preservation of joint chondrocyte survival rate as high as 76% or more, only a small loss of cartilage matrix components. [Conclusion] compared three kinds of methods, tissue culture method can long-term preservation of articular cartilage activity is considered more desirable cartilage preservation. Keywords: articular cartilage; preservation; tissue culture; activity Abstract: [Objective] To study the effects of different storage method on the viability of cheodroeytes so as to find out the idem method for preservation of articular cartilage. [Method] Under the aseptic condition, acquiring 240 pieces of osteoehondral plugs from both condyles of femur and tibial plateau.The control group was not treatment using any preserved means.The tissue  cultured group was to used F12  DMEM medium long  term preserved osteochondral plugs under the conditions of 37 ℃. At preserve 8 weeks, takes the osteochondral section to carry on examination observation ohondrocytes Viability activeness chang