Vitreous body conditioned medium of human retinal pigment epithelial cell growth and cell cycle activity.docVIP

 PAGE \* MERGEFORMAT 23 Vitreous body conditioned medium of human retinal pigment epithelial cell growth and cell cycle activity 【Abstract】 Objective: To explore the conditioned medium containing human vitreous fluid under the action of in vitro cultured human retinal pigment epithelial (HRPE) cell morphology, cell growth activity and cell cycle changes. Methods: HRPE cell cultures containing 100,250,500,1 000mL / L of people vitreous DMEM conditioned medium (VCM). Inverted microscope observation of morphological changes of cells were treated with MTT, flow cytometry cell growth activity and cell cycle changes. Results: Different volume fraction of VCM to HRPE cell morphology changed, HRPE cells, and earlier emergence of multi-core de-pigmentation, similar to fibroblast growth phenotype of the state. With 100,250, and 500mL / L vitreous volume of VCM treated HRPE cells, the growth in activity as compared with the control group there was a significant difference (P all “0.01), containing 250mL / L vitreous VCM capacity to stimulate HRPE cell proliferation most Strong. VCM encourage more HRPE cells entered the DNA synthesis phase (S phase, 6% ~ 13%), with 250 and 500mL / L vitreous volume of VCM to HRPE cells appeared aneuploid changes. Conclusion: a certain volume fraction of VCM to HRPE cells in S-phase fraction increased, the ability to enhance cell proliferation. Keywords: retinal pigment epithelial cells in the vitreous MTT cell cycle by flow cytometry DNA ploidy Effect of vitreous conditional medium on human retinal pigment epithelium cells’ proliferation and cell dividing cycle AbstractAIM: To investigate the change of cellular morphology, cell proliferative activity and cell dividing cycle in human retinal pigment epithelium (HRPE) cells after treatment of human vitreous conditional medium (VCM). METHODS: HRPE cells were treated with VCM including 100, 250, 500 and 1 000mL / L human vitreous respectively. Cellular morphology, c