Xenogeneic blood transfusion - monkey by the Study of red blood cells lose pigs.docVIP

 PAGE \* MERGEFORMAT 31 Xenogeneic blood transfusion - monkey by the Study of red blood cells lose pigs Author: Ying-Xia Tan, Ji Shou-ping, LU Yan-ping, Cheng-Lin Zhang, Li-Li Li, Gong Feng, Zhang Jin, Zhang Yang Pei Abstract The purpose of this study is to transform pig red blood cells (pRBC) surface antigen, so that is compatible with the primates to explore the possibility of heterologous blood transfusion. Combination of α-galactosidase (AGL) digestion, and methoxy polyethylene glycol (mPEG) modified the surface of heterogeneous transformation of porcine red blood cell antigens, and infusion to monkeys, observing monkeys in vivo survival time in pRBC and safety; the use of immunosuppressive agents (CVF venom factor and dexamethasone) to extend the pRBC in the rhesus monkey in vivo survival time; established monkey model of hemorrhagic anemia observed infusion pRBC treatment of hemorrhagic anemia monkeys possibilities. The results showed that: AGL enzyme able to remove the major xenogeneic porcine red blood cell surface antigen (α-Gal antigen), decreased with monkey serum agglutination reaction, enzyme technology and mPEG modification technology could make it more pRBC compared with the monkey serum capacity. Xenogeneic blood transfusion tests showed that pairs of modified pRBC survival time in vivo in monkeys 12 hours after the use of immunosuppressive agents, may be extended to 40 hours; pRBC in the rhesus monkey in vivo 8-hour survival rate was 38% in the pRBC infusion of 8 hours, bleeding monkey hemoglobin and hematocrit can be maintained at the normal level before the loss of blood. Conclusion: The improved pRBC infusion to monkeys can safely improve the symptoms of blood loss anemia monkeys, suggesting that using pig red blood cells to heterologous blood transfusion is possible. Keywords: pig red blood cell Preliminary Study on Xenotransfusion from Porcine Red Blood Cell into Rhesus Monkey Abstract In order to study