Wuling capsule chronic unpredictable mild stress increases hippocampal expression of connexin 43 to improve nerve regeneration.docVIP

 PAGE \* MERGEFORMAT 23 Wuling capsule chronic unpredictable mild stress increases hippocampal expression of connexin 43 to improve nerve regeneration Of: Qiang Li, Li Xujuan, Duan Jinfeng, Cai Wei [Abstract] Objective: To investigate the Wuling Capsule for chronic unpredictable mild stress (chronic unpredictable mild stress, CMS) model of depression in dentate gyrus of brain-derived neurotrophic factor (brain derived neurotrophic factor, BDNF), nerve regeneration , and connexin 43 (connexin 43, Cx43) expression. Methods: 45 adult male Sprague Dawley rats were randomly divided into control group (n = 15), model group (n = 15) and Wuling Capsule group (n = 15). Wuling Capsule model group rats received 3 consecutive weeks of CMS, the modeling period, Wuling Capsule by 100 mg / (kg d) Jiaru Wu Capsules diet rats, continuous treatment of 21 d. Experimental evaluation of using sugar preference degree of depression in rats. After the behavioral tests of depression, take the bilateral hippocampus, detected by immunohistochemistry in BDNF and 5-bromodeoxyuridine (5 bromodeoxyuridine, BrdU) expression by reverse transcription polymerase chain reaction and Western blot detection of the hippocampus in the expression of Cx43 mRNA and protein. Results: Chronic stress in rats dentate gyrus BDNF expression, new cells and the expression of Cx43 mRNA and protein levels significantly decreased compared with normal rats. Ukraine capsule treatment, CMS improved depressive behaviors in rats, abnormal neuronal regeneration recovery, Cx43 mRNA and protein expression became normal, but no significant changes in BDNF expression. Conclusion: The CMS model of Wuling Capsule increased hippocampal nerve regeneration and improvement of depressive symptoms, which may be associated with increased Cx43 expression, and BDNF has nothing to do. [Keywords:] chronic unpredictable mild stress; brain-derived neurotrophic factor; 5 bromodeoxyuridine; junction protein; rat Recently, great