Comparison of Insulin Secretion between Human Adult and Fetal Pancreatic Islets.docVIP

Transplantationsmedizin 2010, 22. Jahrg., S. 176 D. Nikolic et al.: Comparison of Insulin Secretion between Human Adult and Fetal Pancreatic Islets Comparison of Insulin Secretion between Human Adult and Fetal Pancreatic Islets D. Nikolic , P. B. Djordjevic , 1,2 1,2 V. Dimitrijevic-Sreckovic N. Kalezic , B. Nikolic 1,2 , 1,2 2 Background, objectives: The aim of this study was to compare in- sulin secreting capacity of human adult and fetal pancreatic islets in order to establish which one is more suitable for transplantation in IDDM patients. Methods: Both adult and fetal islets were cultured in RPMI 1640 medium for 7 days (short-term cultivation) under conventional stat- ic culture. Insulin secreting capacity was expressed as stimulation index after stimulation with low and high glucose concentration (1.6 mM/L and 16.7 mM/L, respectively). Results: Adult islets had greater stimulation index on day 1 than on day 7 (703.60±2.89% and 72.21±0.58%, respectively), p0.01, in- dicating the loss of functional capacity on the 7th day. Fetal islets, on the other hand, showed slightly increased secretory ability on day 7 compared to day one (395.58±0.12% and 371.52±0.107%, respectively), with statistically significant difference (p0.01). Comparing stimulation indexes of adult and fetal islets on day 1 and day 7, we established that adult islets had greater values on day 1 than fetal islet, p0.05. Fetal islets had greater stimulation index on day 7 than adult ones and difference is statistically significant (p0.001). Conclusions: Based on analyzed results, we concluded that adult islets showed decreased functional ability of insulin secretion in re- sponse to glucose stimulation during 7-day cultivation, while fetal ones had slightly increased secretory capacity under the same con- ditions. These data indicate that fetal tissue cultured in elevated glucose levels is bette