Effects of three controlled mechanical ventilation modes on rat lung hydrogen peroxide and apoptosis during hemorrhagic shock.docVIP

Open Journal of Molecular and Integrative Physiology, 2013, 3, 27-35 OJMIP /10.4236/ojmip.2013.31005 Published Online February 2013 (/journal/ojmip/) Effects of three controlled mechanical ventilation modes on rat lung hydrogen peroxide and apoptosis during hemorrhagic shock Amanda R. Thimmesch, Qiuhua Shen , Richard L. Clancy, Janet D. Pierce * School of Nursing, University of Kansas, Kansas City, USA * Email: qshen@ Received 5 December 2012; revised 10 January 2013; accepted 21 January 2013 ABSTRACT Keywords: Trauma; Animal Model; Lung Injury; Reactive Oxygen Species; Programmed Cell Death Hemorrhagic shock causes a reduction in oxygen supply to tissues leading to increased reactive oxygen species resulting in lung injury. Often mechanical ventilation is required as supportive treatment; how- ever, ventilation can also induce lung injury and apoptosis. The purpose of this study was to examine the effects of three modes of controlled mechanical ventilation: volume control, pressure control, and pressure regulated volume control on lung injury as measured by hydrogen peroxide and apoptosis during hemorrhagic shock. Male Sprague-Dawley rats were randomized to the three controlled mechanical venti- lation groups. Hemorrhagic shock was elicited by removing approximately 40% of the blood volume over 30 minutes. The rats were treated with one of three modes of mechanical ventilation with 40% oxy- gen for 60 minutes. The lungs were removed and measured for hydrogen peroxide and apoptosis based on nuclear differential dye uptake. There were no significant differences in hemodynamics, arterial blood values, peak inspiratory pressures, tidal volume, respiratory rates, and intrathoracic pressures across three groups. However, lung hydrogen peroxide pro- duction and apoptosis were significantly increased in volume control and pressure control, compared to