RSUME、HIF-1αVEGF-A通路与垂体腺瘤侵袭的相关性.pdfVIP

Abstract ABSTRACT Objective: To investigate the functions and correlation of small ubiquitin related modifiers- 1(SUMO- 1) of RWD containing sumoylation enhancer(RSUME) and hypoxia-inducible factor- 1α(HIF- 1α)/vascular endothelial growth factor (VEGF) with pituitary adenoma. To explore the relationship of expression between RSUME, SUMO- 1, HIF- 1 and /VEGF in invasive pituitary adenoma with biological behavior, in order to anti angiogenesis, reduce the invasiveness of pituitary adenomas and provide a new theoretical basis. Methods: 1. To investigate the correlation of expression between RSUME, SUMO- 1, HIF- 1α, VEGF-A in pituitary adenoma and the invasive pituitary adenomas. According to the Hardy-Wilson and Knosp standards and the exam of iconography and the intraoperative findings, pituitary adenomas is divided into invasive pituitary adenomas groups and non- invasive pituitary adenomas groups. The expression of protein and mRNA levels of RSUME, SUMO- 1 , HIF- 1α, VEGF-A was detected by immunohistochemistry, western blot, qPCR in 49 cases of non- invasive pituitary adenoma, 34 cases of invasive pituitary adenomas and 9 normal pituitary capsule, and analysed the expression in different types of pituitary adenomas. 2. To study the mechanism of RSUME and HIF- 1α/VEGF-A pathway in pituitary adenoma After transfecting with siRNA-targeting RSUME into mouse pituitary adenoma AtT-20 cells, the expression levels of RSUME, HIF- 1α, VEGF-A mRNAs and SUMO- 1, HIF- 1α and VEGF-A proteins were detected by real-time fluorescence quantitative-