基于ALA-PDT中PpIX亚细胞分布位点光漂白实验研究.docVIP

Modern Physics 现代物理, 2012, 2, 94-100 /10.12677/mp.2012.24016 Published Online November 2012 (/journal/mp.html) Experimental Research on Subcellular Distribution of PpIX Photobleaching in HL60 Based on ALA-PDT* Kangqiang Huang , Shunfeng Lv , Jianwen Xiong 1,2 2 2# 1 Shantou Branch, Guangdong Co., China Mobile Group, Shantou 2 School of Physics and Telecommunication Engineering, South China Normal University, Guangzhou # Email: xiaoqiang1022@163.com, jwxiong@ Received: Aug. 29 , 2012; revised: Sep. 18 , 2012; accepted: Sep. 30 , 2012 th th th Abstract: With the development of the fundamental research of photodynamic therapy (PDT) and its clinical applica- tion, the photobleaching properties of photosensitizers have become a hotspot of research. The experimental research of ALA mediated PDT was carried out in the own designed reaction chamber when the final concentration of ALA is 10 mM/ml, the wavelength and the output power of the light source are 410 nm and 5 mW/cm separately. Cell Counting 2 Kit-8 (CCK-8) was used to evaluate the cell viability while the photobleaching of PpIX in the subcellular structure of HL60 was detected by fluorescence spectroscopy. The results showed that after 60 min irradiation the fluorescence in- tensity of mitochondria, lysosomes and endoplasmic reticulum had decreased by 95%, 91.5% and 51.2% respectively compared to that before irradiation. The rate of photobleaching in mitochondria which can characterize the activity of HL60 was significantly higher than others, this suggest that mitochondria might be one of main therapeutic targets of photodynamic therapy. Keywords: 5-Aminolaevulinic Acid (ALA); Photodynamic Therapy (PDT); PpIX (Protoporphyrin IX); Photobleaching 基于 ALA-PDT中 PpIX亚细胞分布位点