Application of response surface methodology to the modeling of cellulase purification by solvent extraction英文文献资料.docVIP

Advances in Bioscience and Biotechnology, 2012, 3, 408-416 ABB /10.4236/abb.2012.34058 Published Online August 2012 (http://www.SciRP.org/journal/abb/) Application of response surface methodology to the modeling of cellulase purification by solvent extraction Jagdish Singh , Anamika Sharma * Department of Biotechnology, Mata Gujri College, Fatehgarh Sahib, India * Email: jagdish122@ Received 12 May 2012; revised 27 June 2012; accepted 1 July 2012 ABSTRACT and protozoans [3] and have broad range of applications especially in animal feed, textile, waste water treatment, brewing and wine-making [4-7]. An important obstruc- tion in the exploitation of cellulase is expensive purifica- tion effecting the overall cost of hydrolysis [8-11]. Central composite design (CCD) concerning the puri- fication of cellulase from the Bacillus sp. JS14 in a solvent extraction was established with Response sur- face methodology (RSM). Solvent concentration, pH, temperature and retention time were selected as pro- cess variables to evaluate the purification impact fac- tor in solvent precipitation, including the purification fold and % recovery. An experimental space with 13 purification fold and 23 recovery percentage recovery is achieved through the optimized condition based on the model. The molecular weight of the purified en- zyme was estimated to be 32.5 KDa. Optimum activ- ity of purified enzyme was at pH and temperature 6.5?C and 40?C respectively. Enzyme showed maxi- mum activity with carboxymethyl cellulose as sub- strate with compare to rice husk, wheat straw and sucrose. The purified cellulase activity was inhibited The problems in the purification of enzyme are an im- penetrability in the development of economically feasi- ble bioprocess. Impact of combinatorial interactions of different parameters for the solvent extraction of desired compound is abundant. Response surface methodology (RSM),