Association analyses of single nucleotide polymorphisms in the leptin and leptin receptor genes on milk and morphological traits in Holstein cows英文文献资料.docVIP

Vol.2, No.3, 174-182 (2012) Open Journal of Animal Sciences /10.4236/ojas.2012.23024 Association analyses of single nucleotide polymorphisms in the leptin and leptin receptor genes on milk and morphological traits in Holstein cows Giovanna De Matteis , Maria Carmela Scatà , Francesco Grandoni , Francesca Petrera , 1* 1 1 2 1 1 1 2 Fabio Abeni , Gennaro Catillo , Francesco Napolitano , Bianca Moioli 1 Agricultural Research Council-Animal Production Research Centre (CRA-PCM), Animal Production Research Centre, Via Salaria, Monterotondo, Roma, Italy; Corresponding Author: giovanna.dematteis@entecra.it Agricultural Research Council-Research Centre for Fodder Crops and Dairy Productions (CRA-FLC), Research Centre for Fodder * 2 Crops and Dairy Productions, Via Porcellasco Cremona, Italy Received 2 April 2012; revised 9 May 2012; accepted 17 May 2012 ABSTRACT due to alternative RNA splicing. The isoforms are di- vided into three classes: long, short, and secretory. The long, fully active isoform (LEPR-b) is expressed mainly in the hypothalamus, where it takes part in the energy homeostasis and in the regulation of the activity of the secretory organs. To date, only a few SNP in the leptin receptor gene have been detected and analyzed while the leptin gene has been extensively characterized and several polymorphisms were detected and associated to functional and productive traits in cattle. In this work, for the first time, the bovine leptin receptor gene was fully characterized by sequencing the whole coding region and part of the 5’ flanking region. A group of 95 Holstein cows was genotyped in order to search for SNP at both the LEPR and LEP genes,