Association between P2X7 Receptor Polymorphisms and Bone Status in Mice英文文献资料.docVIP

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Association between P2X7 Receptor Polymorphisms and Bone Status in Mice英文文献资料.doc

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Association between P2X7 Receptor Polymorphisms and Bone Status in Mice英文文献资料

HindawiPublishingCorporation JournalofOsteoporosis Volume2012,ArticleID637986,10pages doi:10.1155/2012/637986 ResearchArticle AssociationbetweenP2X7ReceptorPolymorphismsand BoneStatusinMice SusanneSyberg,1,2PeterSchwarz,1,2SolveigPetersen,1,2ThomasH.Steinberg, 3 Jens-ErikBeckJensen, JenniTeilmann, 2 2andNiklasRyeJ?rgensen 1,2 1 2 3 ResearchCentreofAgeingandOsteoporosis,DepartmentsofDiagnosticsandMedicine,GlostrupUniversityHospital, 2600Glostrup,Denmark OsteoporosisandBoneMetabolicUnit,DepartmentsofEndocrinologyandClinicalBiochemistry,HvidovreUniversityHospital, 2650Hvidovre,Denmark DepartmentofInternalMedicine,WashingtonUniversitySchoolofMedicineandtheSt.LouisVeteransA?airsMedicalCenter, St.Louis,MO63110,USA CorrespondenceshouldbeaddressedtoSusanneSyberg,syberg@ruc.dk Received31March2012;Accepted28May2012 AcademicEditor:ElenaAdinol? Copyright?2012SusanneSybergetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. MacrophagesfrommousestrainswiththenaturallyoccurringmutationP451LinthepurinergicreceptorP2X7haveimpaired responsestoagonists(1).BecauseP2X7receptorsareexpressedinbonecellsandareimplicatedinbonephysiology,weasked whetherstrainswiththeP451Lmutationhaveadi?erentbonephenotype.Bysequencingthemostcommonstrainsofinbredmice, wefoundthatonlyafewstrains(BALB,NOD,NZW,and129)wereharboringthewildallelicversionofthemutation(P451)in thegeneforthepurinergicreceptorP2X7.ThestrainswerecomparedbymeansofdualenergyX-rayabsorptiometry(DXA),bone markers,andthree-pointbending.CulturedosteoclastswereusedintheATP-inducedporeformationassay.Wefoundthatstrains withtheP451allele(BALB/cJand129X1/SvJ)hadstrongerfemursandhigherlevelsoftheboneresorptionmarkerC-telopeptide collagen(CTX)comparedtoC57Bl/6(B6)andDBA/2Jmice.Instrainswiththe451Lallele,pore-formationactivityinosteoclasts invitrowaslowerafterapplicationofATP.Incon