Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems英文文献资料.docVIP

Vol.2, No.5, 444-449 (2010) Natural Science /10.4236/ns.2010.25054 Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems Natalia A. Karnaukhova , Larisa A. Sergievich, Valery N. Karnaukhov * Institute of Cell Biophysics, RussianAcademy of Science, Pushchino, Russian Federation; Corresponding Author: nakarnaukhova@mail.ru * Received 4 December 2009; revised 27 January 2010; accepted 15 March 2010. ABSTRACT defined on the base of fundamental studies of intracellu- lar regulation of metabolism with microspectral lumi- nescent analysis. It formed a new trend in automation of cytodiagnostics. To study plant, animal and microorgan- ism cells both self-luminescence of some intracellular compounds (NADH, flavoproteins, for example) and the secondary luminescence induced by interaction of fluo- rochromes with the cells are used. The advance in fluo- rescent cytodiagnostics is only possible with best meth- ods and tools available for microspectral analysis which present a combination of a fluorescent microscope with a spectroanalysing instrument supplied with electronic registration and control units. Such a system satisfying all needs of cytodiagnostics would consist of the two types of instruments. The interconnection between them is determined by their functional peculiarities which will be considered below with some characteristic parameters using in medicine and ecology [1-3]. Spectral luminescent analysis of single cells and cellular systems enable us to reveal the initial changes of intracellular metabolism that can followed by human diseases or failure in biocenosis. Two cytodiagnostic systems of de- vices and techniques have been developed: 1) Microspectrofluorimeters registering the fluo- rescent spectra of individual cells or intracellu- lar organelles used for fundamental investiga- tions of cell reactions an