Anoikis induction by glycoprotein from Laminaria japonica in HT-29 cells英文文献资料.docVIP

Vol.1, No.2, 49-61 (2011) Open Journal of Preventive Medicine doi:10.4236/ojpm.2011.12008 Anoikis induction by glycoprotein from Laminaria japonica in HT-29 cells Hiroe Go, Hye-Jung Hwang, In-Hye Kim, Taek-Jeong Nam * Department of Food Science and Nutrition, Pukyong National University, Busan, Korea; Corresponding author: namtj@pknu.ac.kr * Received 10 May 2011; revised 1 July 2011; accepted 20 July 2011. ABSTRACT growth of most cells is anchorage-dependent. To main- tain the survival of anchorage-dependent cells, contact with components of the extracellular matrix (ECM) in vivo or contact with hydrophilic, negatively charged sur- faces in vitro are required. Therefore, in the absence of ECM attachment, cell death is induced. This mode of cell-death induction is known as detachment-induced anoikis [1,2]. The term anoikis, a Greek word meaning “homeless- ness,” is a specific type of apoptosis induced by detach- ment from the ECM, loss of cell adhesion, or inappro- priate cell adhesion [3-5]. When epithelial and endothe- lial cells detach from the ECM, these cells normally un- dergo apoptosis; thus, anoikis can suppress the expan- sion of oncogenically transformed cells and allow the maintenance of tissue-cell homeostasis [3-6]. Anoikis has been demonstrated and studied in numerous genu- inely adherent cell types [7]. In contrast to non-trans- formed cells, cancer cells are notoriously able to resist anoikis, and this property has been proposed to contrib- ute to metastasis and new tumor growth beyond the original environment of the cancer cells [8]. Unsurpris- ingly, obtaining resistance to anoikis is a critical step in the metastatic process [2]. Conversely, it may be said that anoikis functions as a physiological barrier to me- tastasis. In this study, we focused on the action induced by LJGP on the cell surface. On the cell surface, there are