Automatic Recognition of Muscle-Invasive T-Lymphocytes Expressing Dipeptidyl-Peptidase IV (CD26) and Analysis of the Associated Cell Surface Phenotypes英文文献资料.docVIP

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Automatic Recognition of Muscle-Invasive T-Lymphocytes Expressing Dipeptidyl-Peptidase IV (CD26) and Analysis of the Associated Cell Surface Phenotypes英文文献资料.doc

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Automatic Recognition of Muscle-Invasive T-Lymphocytes Expressing Dipeptidyl-Peptidase IV (CD26) and Analysis of the Associated Cell Surface Phenotypes英文文献资料

JournalofTheoreticalMedicine,2002Vol.4(1),pp.67–74 AutomaticRecognitionofMuscle-invasiveT-lymphocytes ExpressingDipeptidyl-peptidaseIV(CD26)andAnalysisofthe AssociatedCellSurfacePhenotypes W.SCHUBERTa,b,*,M.FRIEDENBERGERa,R.HAARSa,M.BODEb,L.PHILIPSENb,T.NATTKEMPERcandH.RITTERc aInstituteofMedicalNeurobiology,Molecularpatternrecognitionresearchgroup,Otto-von-GuerickeUniversityofMagdeburg,ZENIT-Building, LeipzigerStr.44,39120Magdeburg,Germany; bMELTECLtd.,ZENIT-Building,LeipzigerStr.44,39120Magdeburg,Germany; cNeuroinformatics group,UniversityofBielefeld,33619Bielefeld,Germany (Received1August2000;In?nalform23April2001) Aneuralcelldetectionsystem(NCDS)fortheautomaticquantitationof?uorescentlymphocytesin tissuesectionswasusedtoanalyzeCD26expressioninmuscle-invasiveT-cells.CD26isacellsurface dipeptidyl-peptidaseIV(DPPIV)involvedinco-stimulatoryactivationofT-cellsandalsoinadhesive events.TheNCDSsystemacquiresvisualknowledgefromasetoftrainingcellimagepatchesselected byauser.Thetrainedsystemevaluatesanimagein2mincalculating(i)thenumber,(ii)thepositions and(iii)thephenotypesofthe?uorescentcells.InthepresentstudywehaveusedtheNCDStoidentity DPPIV(CD26)expressinginvasivelymphocytesinsarcoidmyopathyandtoanalyzetheassociated cellsurfacephenotypes.We?ndhighlyunusualphenotypescharacterizedbydifferentialcombination ofsevencellsurfacereceptorsusuallyinvolvedinco-stimulatoryeventsinT-lymphocytes.Thedata supportadifferentialadhesiveratherthanaco-stimulatoryroleofCD26inmuscle-invasivecells.The adaptabilityoftheNCDSalgorithmtodiversetypesofcellsshouldenableustoapproachanyinvasion process,includinginvasionofmalignantcells. Keywords: Muscle;CD26;Neuralnetwork;Imageanalysis;In?ammatorymyopathy 1. INTRODUCTION proximaltubuliofkidneys(Steinetal.,1989;Hegenetal., 1990; Bu¨hling et al., 1995). DPP IV is implicated in in?ammatoryprocessesandappearstoplayapartinthe progressionofcertainmalignanttumors(Morrisonetal., 1993; Iwata and Morimoto, 1999). Given the cellular immuneresponse(Mor