Arabidopsis thaliana英文文献资料.docVIP

ComparativeandFunctionalGenomics CompFunctGenom2001;2 :91–98. DOI:10.1002/cfg.75 FeaturedOrganism Arabidopsisthaliana JoWixon,ManagingEditor BioinformaticsDivision,HGMP-RC,Hinxton,CambridgeCB101SA,UK Abstract Arabidopsis is universally acknowledged as the model for dicotyledonous crop plants. Furthermore, some of the information gleaned from this small plant can be used to aid work on monocotyledonous crops. Here we provide an overview of the current state of knowledge and resources for the study of this important model plant, with comments on future prospects inthe ?eld from Professor Pamela Green and Dr SeanMay. Copyright #2001JohnWileySons,Ltd. Summary $ $ $ $ $ $ Small?oweringplantofmustardfamily(Brassicaceae) Rapidlifecycle,highseedproduction,easytocultivate Smallgenome,125Mb,as?vechromosomes Genomesequencecompleted,25,498genesidenti?ed 30%ofgenescurrentlyhavenofunctionalclassi?cation Majorityofgenomepresentintwocopies,suggestingwholegenomeduplication Background Toolsforstudy Arabidopsis thaliana was discovered by Johannes Reverse genetic methods for gene knockout in Thal (hence the name thaliana) in the Harz Arabidopsis include various transposon insertion mountains in the sixteenth century. Originally, he systems (such as those based upon the maize Ds called it Pilosella siliquosa, but several name element) and the Agrobacterium tumefaciens trans- changes have occurred since then. This plant, ferred DNA (T-DNA). A range of herbicide commonly known as thale cress, was chosen as a resistance markers is available, including Basta, model for scienti?c research due to the ease with and antibiotic resistance markers for kanamycin, whichitcanbecultivated(manyofthesesmallcress hygromycinandstreptomycinarealsoused.Theb- plants can be grown in a small area), its rapid life glucuronidase (GUS) reporter gene (or uidA gene) cycleandhighproductionofseeds.Anotherfeature has been used to study tissue speci?c expression of this model plant, and