Antimicrobial Activity of Terminalia catappa L. Leaf Extracts against Some Clinically Important Pathogenic Microbial Strains英文文献资料.docVIP

Chinese Medicine, 2011, 2, 171-177 171 doi:10.4236/cm.2011.24027 Published Online December 2011 (http://www.SciRP.org/journal/cm) Antimicrobial Activity of Terminalia catappa L. Leaf Extracts against Some Clinically Important Pathogenic Microbial Strains Sumitra Chanda , Kalpna Rakholiya, Rathish Nair * Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot, India * E-mail: svchanda@ Received July 27, 2011; revised September 2, 2011; accepted September 14, 2011 1 Abstract Objective: The present study was undertaken to evaluate in-vitro antimicrobial activity of methanol, acetone and N, N-dimethylformamide extracts from leaf of Terminalia catappa L. (Combretaceae). Methods: In vi- tro antimicrobial activity of all the extracts was done by agar disc diffusion assay. 91 clinically important strains were used for the study, which were both clinical isolates as well as identified strains. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as stan- dards for antifungal assay. Antimicrobial activity was determined by measurement of inhibition zone around each paper disc. For each extract three replicate trials were conducted against each organism. Results: The antibacterial activity was more pronounced against bacteria than fungal strains. The Gram positive bacteria were more susceptible than Gram negative bacteria. The methanol extract showed best antibacterial activity. T. catappa leaf extracts showed better antibacterial activity than commercially used antibiotics. Conclusion: Demonstration of antimicrobial activity of T. catappa provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antibiotic substances that could serve as selective agents for infectious disease chemotherapy and control. This investigation has opened up the po