Bioinformatics analysis of metallothionein gene from Agaricus bisporus, Ganoderma lucidum, Taiwanofungus camphorates and Paxillus involutus英文文献资料.docVIP

Vol.3, No.7, 900-904 (2012) Agricultural Sciences /10.4236/as.2012.37109 Bioinformatics analysis of metallothionein gene from Agaricus bisporus, Ganoderma lucidum, Taiwanofungus camphoratus and Paxillus involutus Cheng-Kui Qiang , Bao-Ya Zhou , Xiao-Zheng Yang , Feng Wei , Zheng-Li Lu , 1,2 1,2 1,2 2 2 Song-Song Wang 3 1,2* 1,2 , Yue-Hua Qin , Sheng-Yong Wang 1 * 2 3 Xuzhou Key Laboratory of Modern AgroBiotechnology, Xuzhou Vocational College of Bioengineering, Xuzhou, China; Corresponding Author: xzqck@ Department of Agriculture and Landscape Engineering, Xuzhou Vocational College of Bioengineering, Xuzhou, China Office of Science and Education, Xuzhou Agricultural Commission, Xuzhou, China Received 15 July 2012; revised 20 August 2012; accepted 9 October 2012 ABSTRACT and genetic laws. Metallothionein (MT) gene has an important role in the detoxification of toxic metals especially some heavy metals. Based on the published sequences of MT genes from Agaricus bisporus, Garnoderma lucidum, Taiwanofungus campho- ratus and Paxillus involutus as a type of the edible or medical mushroom in NCBI database, their molecular structures, physiological func- tions and evolutionary relationship were ana- lyzed using the bioinformatics methods. Results showed that AbMT, GlMT, TcMT and PiMT genes contained complete open reading frame (ORF) and their theoretical points of the last three en- coding proteins were higher than 7.0. AbMT, GlMT, TcMT and PiMT were relatively rich in random coil and extended strand, but trans- membrane helices and signal peptides were not found. 4 MTs were mainly localized in cell nu- cleus (over 60%) and their cellular functions might h