Bone morphogenetic protein-4 affects both trophoblast and non-trophoblast lineage-associated gene expression in human embryonic stem cells英文文献资料.docVIP

Vol.2, No.4, 163-175 (2012) Stem Cell Discovery /10.4236/scd.2012.24021 Bone morphogenetic protein-4 affects both trophoblast and non-trophoblast lineage-associated gene expression in human embryonic stem cells Margaret L. Shirley , Alison Venable , Raj R. Rao , Nolan L. Boyd , Steven L. Stice , 1,2* 1* 3 4 1,5,6 7# David Puett 1# , Prema Narayan 1 # 2 3 4 5 6 7 # Department of Biochemistry and Molecular Biology, University of Georgia, Athens, USA; Corresponding Author: puett@ Department of Psychiatry, University of California, San Francisco, USA Department of Chemical and Life Science Engineering, School of Engineering, Virginia Commonwealth University, Richmond, USA Cardiovascular Innovation Institute, University of Louisville, Louisville, USA Regenerative Bioscience Center, University of Georgia, Athens, USA Department of Animal and Dairy Sciences, University of Georgia, Athens, USA Department of Physiology, Southern Illinois University School of Medicine, Carbondale, USA; Corresponding Author: pnarayan@ Received 5 May 2012; revised 4 June 2012; accepted 1 July 2012 ABSTRACT cells were obtained. Gene expression by EB was characterized by an up-regulation of a num- ber of genes associated with trophoblast, ecto- derm, endoderm, and mesoderm, and the pro- duction of hCG and progesterone confirmed that trophoblast-like cells were formed. These re- sults suggest that, in the presence of FGF-2, BG02 cells respond to BMP4 to yield tropho- blast-like cells, which are also obtained upon EB formation. Thus, BMP4-mediated differentiation of hESC represents a viable cell system for studying early developmental events post-im- plantation; however, up-regulation of non-tro- phoblast genes suggests a somewhat diverse response to BMP4/FGF-2. Noggin alt