AmyR Is a Novel Negative Regulator of Amylovoran Production in Erwinia amylovora 英文参考文献.docVIP
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AmyR Is a Novel Negative Regulator of Amylovoran Production in Erwinia amylovora 英文参考文献
AmyRIsaNovelNegativeRegulatorofAmylovoran
ProductioninErwiniaamylovora
DongpingWang1,SchuylerS.Korban2,P.LawrencePusey3,YoufuZhao1*
1Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, 2Department of Natural Resources and
Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, 3Tree Fruit Research Laboratory, USDA-Agriculture
ResearchService,Wenatchee,Washington,UnitedStatesofAmerica
Abstract
Inthisstudy,weattemptedtounderstandtheroleofanorphangeneamyRinErwiniaamylovora,afunctionallyconserved
ortholog of ybjN in Escherichia coli, which has recently been characterized. Amylovoran, a high molecular weight acidic
heteropolymer exopolysaccharide, is a virulent factor of E. amylovora. As reported earlier, amylovoran production in an
amyRknockoutmutantwasabouteight-foldhigherthanthatinthewildtype(WT)strainofE.amylovora.Whenamulticopy
plasmid containing the amyR gene was introduced into the amyR mutant or WT strains, amylovoran production was
strongly inhibited. Furthermore, amylovoran production was also suppressed in various amylovoran-over-producing
mutants, such as grrSA containing multicopies of the amyR gene. Consistent with amylovoran production, an inverse
correlationwasobservedbetweeninvitroexpressionofamyRandthatofamylovoranbiosyntheticgenes.However,both
the amyR knockout mutant and over-expression strains showed reduced levan production, another exopolysaccharide
produced by E. amylovora. Virulence assays demonstrated that while the amyR mutant was capable of inducing slightly
greaterdiseaseseveritythanthatoftheWTstrain,strainsover-expressingtheamyRgenedidnotincitediseaseonapple
shootsorleaves,andonlycausedreduceddiseaseonimmaturepearfruits.Microarraystudiesrevealedthatamylovoran
biosynthesis and related membrane protein-encoding genes were highly expressed in the amyR mutant, but down-
regulatedintheamyRover-expressionstrainsinvitro.Down-re
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