Arrhythmia Caused by a Drosophila Tropomyosin Mutation Is Revealed Using a Novel Optical Coherence Tomography Instrument 英文参考文献.docVIP

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Arrhythmia Caused by a Drosophila Tropomyosin Mutation Is Revealed Using a Novel Optical Coherence Tomography Instrument 英文参考文献.doc

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Arrhythmia Caused by a Drosophila Tropomyosin Mutation Is Revealed Using a Novel Optical Coherence Tomography Instrument 英文参考文献

ArrhythmiaCausedbyaDrosophilaTropomyosin MutationIsRevealedUsingaNovelOpticalCoherence TomographyInstrument LishaMa1*¤,AdrianBradu2,AdrianGh.Podoleanu2,JamesW.Bloor1 1Cell and Developmental Biology Group, School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom, 2Applied Optics Group, School of Physical Sciences,UniversityofKent,Canterbury,Kent,UnitedKingdom Abstract Background:Dilatedcardiomyopathy(DCM)isaseverecardiacconditionthatcauseshighmortality.Manygeneshavebeen confirmedtobeinvolvedinthisdisease.Anidealsystemwithwhichtouncoverdiseasemechanismswouldbeonethatcan measurethechangesinawiderangeofcardiacactivitiesassociatedwithmutationsinspecific,diverselyfunctionalcardiac genes. Such a system needs a genetically manipulable model organism that allows in vivo measurement of cardiac phenotypesandadetectinginstrumentcapableofrecordingmultiplephenotypeparameters. MethodologyandPrincipalFindings:Withasimpleheart,atransparentbodysurfaceatlarvalstagesandavailablegenetic tools we chose Drosophila melanogaster as our model organism and developed for it a dual en-face/Doppler optical coherencetomography(OCT)instrumentcapableofrecordingmultipleaspectsofheartactivity,includingheartcontraction cycle dynamics, ostia dynamics, heartbeat rate and rhythm, speed of heart wall movement and light reflectivity of cardiomyocytesinsitu.WeappliedthisOCTinstrumenttoamodelofTropomyosin-associatedDCMestablishedinadult Drosophila.WeshowthatDCMpre-existsinthelarvalstageandisaccompaniedbyanarrhythmiapreviouslyunidentifiedin thismodel.Wealsodetectreducedmobilityandlightreflectivityofcardiomyocytesinmutants. Conclusion: These results demonstrate the capability of our OCT instrument to characterize in detail cardiac activity in geneticmodelsforheartdiseaseinDrosophila. Citation:MaL,BraduA,PodoleanuAG,BloorJW(2010)ArrhythmiaCausedbyaDrosophilaTropomyosinMutationIsRevealedUsingaNovelOpticalCoherence TomographyInstrument.PLoSONE5(12):e14348.doi:10.1371/journal.pone.0014348 Editor