Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material 英文参考文献.docVIP

EffectsofFeedingBtMaizetoSowsduringGestationand LactationonMaternalandOffspringImmunityandFate ofTransgenicMaterial StefanG.Buzoianu1,2,MariaC.Walsh1,MaryC.Rea3,4,OrlaO’Donovan2,EvaGelencse′r5, GabriellaUjhelyi5,ErikaSzabo′5,AndrasNagy5,R.PaulRoss3,4,GillianE.Gardiner2,PeadarG.Lawlor1* 1Teagasc,PigDevelopmentDepartment,AnimalandGrasslandResearchandInnovationCentre,Moorepark,Fermoy,Co.Cork,Ireland,2DepartmentofChemicaland LifeSciences,WaterfordInstituteofTechnology,Waterford,Co.Waterford,Ireland,3TeagascFoodResearchCentre,Moorepark,Fermoy,Co.Cork,Ireland,4Alimentary PharmabioticCentre,UniversityCollegeCork,Cork,Co.Cork,Ireland,5CentralFoodResearchInstitute,Budapest,Hungary Abstract Background: We aimed to determine the effect of feeding transgenic maize to sows during gestation and lactation on maternalandoffspringimmunityandtoassessthefateoftransgenicmaterial. Methodology/Principal Findings: On the day of insemination, sows were assigned to one of two treatments (n=12/ treatment);1)non-Btcontrolmaizedietor2)Bt-MON810maizediet,whichwerefedfor,143daysthroughoutgestation and lactation. Immune function was assessed by leukocyte phenotyping, haematology and Cry1Ab-specific antibody presence in blood on days 0, 28 and 110 of gestation and at the end of lactation. Peripheral-blood mononuclear cell cytokineproductionwasinvestigatedondays28and110ofgestation.Haematologicalanalysiswasperformedonoffspring at birth (n=12/treatment). Presence of the cry1Ab transgene was assessed in sows’ blood and faeces on day 110 of gestation and in blood and tissues of offspring at birth. Cry1Ab protein presence was assessed in sows’ blood during gestationandlactationandintissuesofoffspringatbirth.Bloodmonocytecountandpercentagewerehigher(P,0.05), while granulocyte percentage was lower (P,0.05) in Bt maize-fed sows on day 110 of gestation. Leukocyte count and granulocytecountandpercentagewerelower(P,0.05),whilelymphocytepercentagewashigher(P,0.05)inoffspringof + + Bt maize-fed sows. Bt maize