Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits 英文参考文献.docVIP

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Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits 英文参考文献.doc

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Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits 英文参考文献

EffectoftheCitrusLycopeneb-CyclaseTransgeneon CarotenoidMetabolisminTransgenicTomatoFruits FeiGuo1,WenjingZhou1,JianchengZhang1,QiangXu1,2,XiuxinDeng1,2 * 1Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, China, 2National Key Laboratory of Crop Genetic Improvement,HuazhongAgriculturalUniversity,Wuhan,China Abstract Lycopeneb-cyclase(LYCB)isthekeyenzymeforthesynthesisofb-carotene,avaluablecomponentofthehumandiet.In thisstudy,tomatoconstitutivelyexpressLycb-1wasengineered.Theb-caroteneleveloftransformantincreased4.1fold,and the total carotenoid content increased by 30% in the fruits. In the transgenic line, the downstream a-branch metabolic fluxes were repressed during the three developmental stages while a-carotene content increased in the ripe stage. Microarray analysis in the ripe stage revealed that the constitutive expression of Lycb-1 affected a number of pathways includingthesynthesisoffattyacids,flavonoidsandphenylpropanoids,thedegradationoflimoneneandpinene,starch andsucrosemetabolismandphotosynthesis.ThisstudyprovidedinsightintotheregulatoryeffectofLycb-1geneonplant carotenoidmetabolismandfruittranscriptome. Citation:GuoF,ZhouW,ZhangJ,XuQ,DengX(2012)EffectoftheCitrusLycopene b-CyclaseTransgeneonCarotenoidMetabolisminTransgenicTomato Fruits.PLoSONE7(2):e32221.doi:10.1371/journal.pone.0032221 Editor:RandallP.Niedz,UnitedStatesDepartmentofAgriculture,UnitedStatesofAmerica ReceivedOctober21,2011;AcceptedJanuary22,2012;PublishedFebruary24,2012 Copyright:?2012Guoetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbytheMinistryofScienceandTechnologyofChina(Nos.2011CB100601,2011AA100205)andtheNationalNaturalScience FoundationofChina(No.Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisi