Electrophysiology of Single and Aggregate Cx43 Hemichannels 英文参考文献.docVIP

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Electrophysiology of Single and Aggregate Cx43 Hemichannels 英文参考文献.doc

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Electrophysiology of Single and Aggregate Cx43 Hemichannels 英文参考文献

ElectrophysiologyofSingleandAggregateCx43 Hemichannels ColeBrokamp,JacobTodd,CarloMontemagno*,DavidWendell* SchoolofEnergy,Environmental,BiologicalandMedicalEngineering,EngineeringResearchCenter,UniversityofCincinnati,Cincinnati,Ohio,UnitedStatesofAmerica Abstract Connexin43 (Cx43) is the most ubiquitous gap junction protein in the human body and is essential for cell-to-cell communicationinavarietyoforgansandorgansystems.Asaresult,Cx43isresponsibleformediatingbothelectricaland chemicalsignals,passingdissolvedsolutesandsmallsignalingmoleculesbetweencellsinacoordinatedfashion.Here,we explore theelectrophysiological properties of hemichannels formed fromCx43 andCx43fused toeGFP (Cx43eGFP) and theirinteractionsinaplanarlipidmembrane(BLM).Unlikeinvivopatchclampexperiments,Cx43waspurifiedandisolated fromothermembraneconstituentsallowingelucidationofindividualproteinresponsestovariouselectricalandchemical stimuli.Usingthissystem,weexaminedhemichannelelectrophysiologyandtherolesofseveralwell-knowngapjunction blockers,namely:lanthanum,heptanol,carbenoxaloneandlindane.Wealsoobservedacriticalnumberofhemichannels requiredforanacceleratedconductanceincrease,anemergentelectricalsignatureindicativeofplaqueformation. Citation: Brokamp C, Todd J, Montemagno C, Wendell D (2012) Electrophysiology of Single and Aggregate Cx43 Hemichannels. PLoS ONE 7(10): e47775. doi:10.1371/journal.pone.0047775 Editor:XiongwenChen,TempleUniversity,UnitedStatesofAmerica ReceivedJuly16,2012;AcceptedSeptember17,2012;PublishedOctober24,2012 Copyright: ? 2012 Brokamp et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: Thisworkwassponsoredbyinternalfunds fromtheUniversityofCincinnati. Thefunderhadnoroleinstudydesign,datacollectionandanalysis, decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthor