Endogenous TasiRNAs Mediate Non-Cell Autonomous Effects on Gene Regulation in Arabidopsis thaliana 英文参考文献.docVIP

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Endogenous TasiRNAs Mediate Non-Cell Autonomous Effects on Gene Regulation in Arabidopsis thaliana 英文参考文献.doc

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Endogenous TasiRNAs Mediate Non-Cell Autonomous Effects on Gene Regulation in Arabidopsis thaliana 英文参考文献

EndogenousTasiRNAsMediateNon-CellAutonomous EffectsonGeneRegulationinArabidopsisthaliana RebeccaSchwab1.¤a,AlexisMaizel2.,VirginiaRuiz-Ferrer3.,DamienGarcia1¤a,MartinBayer1¤b ,Martin Crespi2,OlivierVoinnet3,RobertA.Martienssen1* 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America, 2Institut des Sciences du Ve′ge′tal, CNRS UPR2355, Gif-sur-Yvette, France, 3InstitutdeBiologieMole′culairedesPlantes,CNRSUPR2353,Universite′ LouisPasteur,Strasbourg,France Abstract Background: Different classes of small RNAs (sRNAs) refine the expression of numerous genes in higher eukaryotes by directingproteinpartnerstocomplementarynucleicacids,wheretheymediategenesilencing.Plantsencodeauniqueclass of sRNAs, called trans-acting small interfering RNAs (tasiRNAs), which post-transcriptionally regulate protein-coding transcripts, as do microRNAs (miRNAs), and both sRNA classes control development through their targets. TasiRNA biogenesisrequiresmultiplecomponentsofthesiRNApathwayandalsomiRNAs.Butwhile21mersiRNAsoriginatingfrom transgenes can mediate silencing across several cell layers, miRNA action seems spatially restricted to the producing or closelysurroundingcells. Principal Findings: We have previously described the isolation of a genetrap reporter line for TAS3a, the major locus producingAUXINRESPONSFACTOR(ARF)-regulatingtasiRNAsintheArabidopsisshoot.Itsactivityislimitedtotheadaxial (upper)sideofleafprimordia,thusspatiallyisolatedfromARF-activities,whicharelocatedintheabaxial(lower)side.We showherebyinsituhybridizationandreporterfusionsthatthesilencingactivitiesofARF-regulatingtasiRNAsareindeed manifestednon-cellautonomouslytospatiallycontrolARFactivities. Conclusions/Significance:EndogenoustasiRNAsarethusmediatorsofamobiledevelopmentalsignalandmightprovide effectivegenesilencingatadistancebeyondthereachofmostmiRNAs. Citation:SchwabR,MaizelA,Ruiz-FerrerV,GarciaD,BayerM,etal.(2009)EndogenousTasiRNAsMediateNon-CellAutonomousEffectsonGeneRegulat