Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging 英文参考文献.docVIP

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Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging 英文参考文献.doc

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Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging 英文参考文献

EnhancedBeetleLuciferaseforHigh-Resolution BioluminescenceImaging YoshihiroNakajima1*,TomomiYamazaki2,ShigeakiNishii2,TakakoNoguchi1,HidetoHoshino1 ,Kazuki Niwa1,VadimR.Viviani3,YoshihiroOhmiya1* 1NationalInstituteofAdvancedIndustrialScienceandTechnology(AIST),Ikeda,Osaka,Japan,2TsurugaInstituteofBiotechnology,TOYOBOCo.,Ltd.,Tsuruga,Fukui, Japan,3Laborato′riodeBioqu?′micaeBiotecnologiadeSistemasBioluminescentes,UniversidadeFederaldeSa?o Carlos,CampusdeSorocaba,Sorocaba,Sa?o Paulo,Brazil Abstract Wedevelopedanenhancedgreen-emittingluciferase(ELuc)tobeused asabioluminescenceimaging(BLI)probe.ELuc exhibitsalightsignalinmammaliancellsthatisover10-foldstrongerthanthatofthefireflyluciferase(FLuc),whichisthe mostwidelyusedluciferasereportergene.WeshowedthatELucproducesastronglightsignalinprimarycellsandtissues andthatitenablesthevisualizationofgeneexpressionwithhightemporalresolutionatthesingle-celllevel.Moreover,we successfully imaged thenucleocytoplasmic shuttling ofimportin a by fusingELuc at theintracellular level.Theseresults demonstratethattheuseofELucallowsaBLIspatiotemporalresolutionfargreaterthanthatprovidedbyFLuc. Citation:NakajimaY,YamazakiT,NishiiS,NoguchiT,HoshinoH,etal.(2010)EnhancedBeetleLuciferaseforHigh-ResolutionBioluminescenceImaging.PLoS ONE5(4):e10011.doi:10.1371/journal.pone.0010011 Editor:ImmoA.Hansen,NewMexicoStateUniversity,UnitedStatesofAmerica ReceivedJuly29,2009;AcceptedMarch15,2010;PublishedApril2,2010 Copyright: ? 2010 Nakajima et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisstudywassupportedbyaGrant-in-AidforScientificResearch(NooYN)fromJapanSocietyforthePromotionofScience(JSPS),New EnergyandIndustrialTechnologyDevelopmentOrganization(NEDO),andFundac?a?o deAmparoAPesquisadoEstadodeSa?o Paulo(FAPESP,Brazil)forfunding thebasi