Entomopathogenic Fungi on Hemiberlesia pitysophila 英文参考文献.docVIP

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Entomopathogenic Fungi on Hemiberlesia pitysophila 英文参考文献.doc

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Entomopathogenic Fungi on Hemiberlesia pitysophila 英文参考文献

EntomopathogenicFungionHemiberlesiapitysophila ChengqunLv*,BaolingHuang,MengjiQiao,JiguangWei,BoDing ForestryCollege,GuangxiUniversity,Nanning,China Abstract HemiberlesiapitysophilaTakagiisanextremelyharmfulexoticinsectinforesttoPinusspecies,includingPinusmassoniana. Usingbothmorphologicaltaxonomyandmolecularphylogenetics,weidentified15strainsofentomogenousfungi,which belong to 9 genera with high diversities. Surprisingly, we found that five strains that were classified as species of Pestalotiopsis,whichhasbeenconsideredplantpathogensandendophytes,werethedominantentomopathogenicfungus of H. pitysophila. Molecular phylogenetic tree established by analyzing sequences of ribosomal DNA internal transcribed spacershowedthatentomopathogenicPestalotiopsisspp.weresimilartoplantPestalotiopsis,butnottootherpathogens andendophytesofitshostplantP.massoniana.WewerethefirsttoisolateentomopathogenicPestalotiopsisspp.fromH. pitysophila.OurfindingssuggestapotentialandpromisingmethodofH.pitysophilabio-control. Citation: Lv C, Huang B, Qiao M, Wei J, Ding B (2011) Entomopathogenic Fungi on Hemiberlesia pitysophila. PLoS ONE 6(8): e23649. doi:10.1371/ journal.pone.0023649 Editor:GuySmagghe,GhentUniversity,Belgium ReceivedFebruary28,2011;AcceptedJuly22,2011;PublishedAugust25,2011 Copyright:?2011Lvetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbytheGuangxiForestryScienceFoundationofChina(2008,No.01).Thefundershadnoroleinstudydesign,datacollection andanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:luchengqun8@126.com Introduction oneitherentomogenousorentomopathogenicfungiofHemiberlesia pitysophilaTakagi. Insect pathogenic fungi or entomopathogenic fungi (EPF) are thefungicapableofinvading,parasitizing,andcausin