Estimating Fitness by Competition Assays between Drug Susceptible and Resistant Mycobacterium tuberculosis of Predominant Lineages in Mumbai, India 英文参考文献.docVIP

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Estimating Fitness by Competition Assays between Drug Susceptible and Resistant Mycobacterium tuberculosis of Predominant Lineages in Mumbai, India 英文参考文献.doc

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Estimating Fitness by Competition Assays between Drug Susceptible and Resistant Mycobacterium tuberculosis of Predominant Lineages in Mumbai, India 英文参考文献

EstimatingFitnessbyCompetitionAssaysbetweenDrug SusceptibleandResistantMycobacteriumtuberculosisof PredominantLineagesinMumbai,India PurvaBhatter,AnirvanChatterjee,DesireeD’souza,MonicaTolani,NergesMistry* TheFoundationforMedicalResearch,Mumbai,Maharashtra,India Abstract Background:MultiDrugResistantTuberculosis(MDRTB)isathreattoglobaltuberculosiscontrol.Asignificantfitnesscost hasbeenassociatedwithDRstrainsfromspecificlineages.Evaluationoftheinfluenceofthecompetingdrugsusceptible strainsonfitnessofdrugresistantstrainsmayhaveanimportantbearingonunderstandingthespreadofMDRTB.Theaim ofthisstudywastoevaluatethefitnessofMDRTBstrains,fromaTBendemicregionofwesternIndia:Mumbai,belonging to 3 predominant lineages namely CAS, Beijing and MANU in the presence of drug susceptible strains from the same lineages. Methodology:Drugsusceptiblestrainsfromasinglelineageweremixedwithdrugresistantstrain,bearingparticularnon synonymous mutation (rpoB D516V; inhA, A16G; katG, S315T1/T2) from the same or different lineages. Fitness of M.tuberculosis(M.tb)strainswasevaluatedusingthedifferenceingrowthratesobtainedbyusingtheCFUassaysystem. Conclusion/Significance:WhileMANUweremostfitamongstthedrugsusceptiblestrainsofthe3lineages,onlyBeijingMDR strainswerefoundtogrowinthepresenceofanyofthecompetingdrugsusceptiblestrains.Adisproportionateincreasein Beijing MDR could be an alarm for an impending epidemic in this locale. In addition to particular non synonymous substitutions,thecompetingstrainsinanenvironmentmayimpactthefitnessofcirculatingdrugresistantstrains. Citation: Bhatter P, Chatterjee A, D’souza D, Tolani M, Mistry N (2012) Estimating Fitness by Competition Assays between Drug Susceptible and Resistant MycobacteriumtuberculosisofPredominantLineagesinMumbai,India.PLoSONE7(3):e33507.doi:10.1371/journal.pone.0033507 Editor:TanyaParish,QueenMaryUniversityofLondon,UnitedKingdom ReceivedJuly7,2011;AcceptedFebruary15,2012;PublishedMarch27,2012 Copyright: ? 2012 Bhatter et al. This is an open-