Genome-Wide Analysis of Heteroduplex DNA in Mismatch Repair–Deficient Yeast Cells Reveals Novel Properties of Meiotic Recombination Pathways 英文参考文献.docVIP
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Genome-Wide Analysis of Heteroduplex DNA in Mismatch Repair–Deficient Yeast Cells Reveals Novel Properties of Meiotic Recombination Pathways 英文参考文献
Genome-WideAnalysisofHeteroduplexDNAin
MismatchRepair–DeficientYeastCellsRevealsNovel
PropertiesofMeioticRecombinationPathways
EmmanuelleMartini1*,Vale′rieBorde2,3,MatthieuLegendre4,Ste′phaneAudic4,5,Be′atriceRegnault6,
GuillaumeSoubigou6,BernardDujon7,BertrandLlorente7,8*
1CEADSV/IRCM,Unite′ MixtedeRecherche217RadiobiologieMole′culaireetCellulaire,CentreNationaldelaRechercheScientifique,Commissariata` l’EnergieAtomique
et aux Energies Alternatives, Fontenay aux Roses, France, 2Unite′ Mixte de Recherche 218, Centre National de la Recherche Scientifique, Paris, France, 3Centre de
Recherche,InstitutCurie,Paris,France,4Unite′ Proprederecherche2589,StructuralandGenomicInformationLaboratory,CentreNationaldelaRechercheScientifique,
Mediterranean Institute of Microbiology IFR88, Aix-Marseille University, Parc Scientifique de Luminy, Marseille, France, 5UMR 7144, Adaptation et Diversite′ en Milieu
Marin,EquipeEvolutionduPlanctonetPale′o-Oce′ans, StationBiologiquedeRoscoff,CentreNationaldelaRechercheScientifiqueandUniversityPierreandMarieCurie-
Paris, Roscoff, France, 6Ge′nopole, Institut Pasteur, Paris, France, 7Unite′ de Ge′ne′tique Mole′culaire des Levures, Institut Pasteur, Centre National de la Recherche
Scientifique/UniversityPierreandMarieCurie-Paris,Paris,France,8Unite′ PropredeRecherche3081,LaboratoryofGenomeInstabilityandCarcinogenesis,conventionne′
parl’Universite′ d’Aix-Marseille2,CentreNationaldelaRechercheScientifique,Marseille,France
Abstract
Meiotic DNA double-strand breaks (DSBs) initiate crossover (CO) recombination, which is necessary for accurate
chromosome segregation, but DSBs may also repair as non-crossovers (NCOs). Multiple recombination pathways with
specificintermediatesareexpectedtoleadtoCOsandNCOs.WerevisitedthemechanismsofmeioticDSBrepairandthe
regulation of CO formation, by conducting a genome-wide analysis of strand-transfer intermediates associated with
recombinationevents.WeperformedthisanalysisinaSK16S288CSaccharomycescer
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