Mesenchymal Stem Cell Therapy Regenerates the Native Bone-Tendon Junction after Surgical Repair in a Degenerative Rat Model 英文参考文献.docVIP

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Mesenchymal Stem Cell Therapy Regenerates the Native Bone-Tendon Junction after Surgical Repair in a Degenerative Rat Model 英文参考文献.doc

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Mesenchymal Stem Cell Therapy Regenerates the Native Bone-Tendon Junction after Surgical Repair in a Degenerative Rat Model 英文参考文献

MesenchymalStemCellTherapyRegeneratestheNative Bone-TendonJunctionafterSurgicalRepairina DegenerativeRatModel GeoffroyNourissat1,AmadouDiop2,NathalieMaurel2,ColetteSalvat1,SylvieDumont3 ,Audrey Pigenet1,MarjolaineGosset1,XavierHouard1,FrancisBerenbaum1,4* 1UR 4, Pierre Marie Curie University, Paris, France, 2Biomechanics and bone remodeling (EPBRO), Arts et Me′tiers ParisTech, Paris, France, 3Department of anatomopathology,AP-HPSaintAntoinehospital,PierreMarieCurieUniversity,Paris,France,4DepartmentofRheumatology,AP-HPSaintAntoinehospital,Pierre MarieCurieUniversity,Paris,France Abstract Background: The enthesis, which attaches the tendon to the bone, naturally disappears with aging, thus limiting joint mobility.Surgeryisfrequentlyneededbuttheclinicaloutcomeisoftenpoorduetothedecreasednaturalhealingcapacity oftheelderly.Thisstudyexploredthebenefitsofatreatmentbasedoninjectingchondrocyteandmesenchymalstemcells (MSC)inanewratmodelofdegenerativeenthesisrepair. Methodology: The Achilles’ tendon was cut and the enthesis destroyed. The damage was repaired by classical surgery withoutcellinjection(groupG1,n=52)andwithchondrocyte(groupG2,n=51)orMSCinjection(groupG3,n=39).The healingratewasdeterminedmacroscopically15,30and45dayslater.Theproductionandorganizationofanewenthesis was assessed by histological scoring of collagen II immunostaining, glycoaminoglycan production and the presence of columnarchondrocytes.Thebiomechanicalloadrequiredtorupturethebone-tendonjunctionwasdetermined. PrincipalFindings:ThespontaneoushealingrateintheG1controlgroupwas40%,closetothoseobservedinhumans.Cell injectionsignificantlyimprovedhealing(69%,p=0.0028forG2andp=0.006forG3)andtheload-to-failureafter45days (p,0.05)overcontrols.Anewenthesiswasclearlyproducedincell-injectedG2andG3rats,butnotinthecontrols.Onlythe MSC-injectedG3ratshadanorganizedenthesiswithcolumnarchondrocytesasinanativeenthesis45daysaftersurgery. Conclusions: Cell therapy is an efficient procedure for reconstructing degenerativ