O2 Level Controls Hematopoietic Circulating Progenitor Cells Differentiation into Endothelial or Smooth Muscle Cells 英文参考文献.docVIP
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O2 Level Controls Hematopoietic Circulating Progenitor Cells Differentiation into Endothelial or Smooth Muscle Cells 英文参考文献
O2LevelControlsHematopoieticCirculatingProgenitor
CellsDifferentiationintoEndothelialorSmoothMuscle
Cells
NicolasBerthelemy1.,HalimaKerdjoudj1.,PierreSchaaf2,ChristinePrin-Mathieu3,PatrickLacolley4,
Jean-Franc?oisStoltz1,Jean-ClaudeVoegel5,PatrickMenu1*
1GroupofBioengineering(UMRCNRS7561),UHP-Nancy1,Faculte′ deMe′decine,Vandoeuvre-le`s-Nancy, France,2InstitutCharlesSadron(UPR22,CNRS),Strasbourg,
France,3CHUNancyandUHP-Nancy1,Faculte′ deMe′decine,Vandoeuvre-le`s-Nancy, France,4InstitutNationaldelaSante′ etdelaRechercheMe′dicale,(INSERMUnite′
961),UHP-Nancy1,Faculte′ deMe′decine,Vandoeuvre-le`s-Nancy,France,5InstitutNationaldelaSante′ etdelaRechercheMe′dicale,(INSERMUnite′ 977),Universite′ de
Strasbourg,Faculte′ dechirurgiedentaire,Strasbourg,France
Abstract
Background: Recent studies showed that progenitor cells could differentiate into mature vascular cells. The main
physiologicalfactorsimplicatedincelldifferentiationarespecificgrowthfactors.Wehypothesizedthatsimplybyvarying
the oxygen content, progenitor cells can be differentiated either in mature endothelial cells (ECs) or contractile smooth
musclecells(SMCs)whilekeepingexactlythesameculturemedium.
Methodology/PrincipalFindings:Mononuclearcellswereisolatedbydensitygradientwerecultivatedunderhypoxic(5%
O2) or normoxic (21% O2) environment. Differentiated cells characterization was performed by confocal microscopy
examination and flow cytometry analyses. The phenotype stability over a longer time period was also performed. The
morphological examination of the confluent obtained cells after several weeks (between 2 and 4 weeks) showed two
distinctmorphologies:cobblestoneshapeinnormoxiaandaspindlelikeshapeinhypoxia.Thecellcharacterizationshowed
thatcobblestonecellswerepositivetoECsmarkerswhilespindlelikeshapecellswerepositivetocontractileSMCsmarkers.
Moreover, after several further amplification (until 3rd passage) in hypoxic or normoxic conditions of the previously
differentiated SMC, immunoflu
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