Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing 英文参考文献.docVIP

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Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing 英文参考文献.doc

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Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing 英文参考文献

Open Access M d 2003CastleetVoal.lumeetho4, Issue 10, Article R66 Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing ¤ ¤ John Castle , Phil Garrett-Engele , Christopher D Armour, Sven J Duenwald, Patrick M Loerch, Michael R Meyer, Eric E Schadt, Roland Stoughton, Mark L Parrish, Daniel D Shoemaker and Jason M Johnson Address: Rosetta Inpharmatics, Merck Co. Inc., 12040 115th Ave NE, Kirkland, Washington 98034, USA. ¤ These authors contributed equally to this work. Correspondence: Jason M Johnson. E-mail: jason_johnson@ Published: 19 September 2003 Received: 30 May 2003 Revised: 25 July 2003 Accepted: 15 August 2003 Genome Biology 2003, 4:R66 The electronic version of this article is the complete one and can be found online at /2003/4/10/R66 ? 2003 Castle et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. OptimizationfMarvoiamtrirhcpihmraloitigsfiaohrwcn-rteashryaersnoooudpofpfgftirhesiooligonucleotidemorptfauoiozcthreomdidlgistfhmohc-orarixvetdtespeuorteyrleruecmosttfi,arraysioioamtnsnltlmeoaprfbenteeaandhaxlnteioinsvntgifesRNAso,osrefupxmeloincosnamplificationtipnni-reignetc.oittfrriiocanningtysepcdorrgfiepek-stmprotocolsns,.oaRAwnNdlnsAoehx,suohpmnylforibcajuinninanalysisdgacilztotiaenotrninaosang.teTiofcvnhoeoentranscriptmisdseoiitcofioposrcntmiasml,seip,.zstructurearWeondbdepehprcoarhovtaoevrcidanddoceelstveaecralternativelarioosnptbibecuedss,uta,asnsecodavtlasplicingel,aonbdalunbiasedelysistpeclattalgo-smallform Abstract Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteri