Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs 英文参考文献.docVIP

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Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs 英文参考文献.doc

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Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs 英文参考文献

Acta vet. scand. 2001, 42, 279-286. Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs By I. Saeed1, A. Roepstorff1, T. Rasmussen1,2, M. H?g1, and G. Jungersen2,3 1Danish Centre For Experimental Parasitology, 2Department of Clinical Studies, The Royal Veterinary and Agri- cultural University, Frederiksberg, and 3Danish Veterinary Laboratory, Copenhagen, Denmark. Saeed I, Roepstorff A, Rasmussen T, H?g M, Jungersen G: Optimization of the agar-gel method for isolation of migrating Ascaris suum larvae from the liver and lungs of pigs. Acta vet. scand. 2001, 42, 279-286. – Experiments on use of an agar-gel method for recovery of migrating Ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. Subsamples of blended tissues of pig liver and lungs were mixed with agar to a ?nal concentration of 1% agar and the lar- vae allowed to migrate out of the agar-gel into 0.9% NaCl at 38°C. The results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agar- gel and more than 83% of the obtained larvae migrated out of the lung agar-gel. The lar- vae were subsequently available in a very clean suspension which reduced the sample counting time. Blending the liver for 60 sec in a commercial blender showed signi?- cantly higher larvae recovery than blending for 30 sec. Addition of gentamycin to re- duce bacterial growth during incubation, glucose to increase larval motility during mi- gration or ice to increase sedimentation of migrated larvae did not in?uence larvae recovery signi?cantly. Ascaris suum; larva recovery; agar-gel method; liver; lungs. Introduction Simple microscopical quanti?cation of Ascaris suum larvae migrating in the liver and lungs of pigs as carried out by e.g. Dourvres et al. (1969) is very labourous, while the use of a modi?ed Baermann technique on blended tis- sue (