Population Structure of Invasive Streptococcus pneumoniae in the Netherlands in the Pre-Vaccination Era Assessed by MLVA and Capsular Sequence Typing 英文参考文献.docVIP
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Population Structure of Invasive Streptococcus pneumoniae in the Netherlands in the Pre-Vaccination Era Assessed by MLVA and Capsular Sequence Typing 英文参考文献
PopulationStructureofInvasiveStreptococcus
pneumoniaeintheNetherlandsinthePre-Vaccination
EraAssessedbyMLVAandCapsularSequenceTyping
KarinE.M.Elberse1*,IngridvandePol1,SandraWitteveen1,HanG.J.vanderHeide1,CorrieS.Schot1,
AnitavanDijk1,ArievanderEnde2,LeoM.Schouls1
1LaboratoryforInfectiousDiseasesandPerinatalScreening,NationalInstituteforPublicHealthandtheEnvironment(RIVM),Bilthoven,TheNetherlands,2Departmentof
MedicalMicrobiologyandtheNetherlandsReferenceLaboratoryforBacterialMeningitis,AcademicMedicalCenter,Amsterdam,TheNetherlands
Abstract
Theintroductionofnationwidepneumococcalvaccinationmayleadtoserotypereplacementandtheemergenceofnew
variantsthathaveexpandedtheirgeneticrepertoirethroughrecombination.Tomonitoralterationsinthepneumococcal
population structure, we have developed and utilized Capsular Sequence Typing (CST) in addition to Multiple-Locus
Variable number tandem repeat Analysis (MLVA). To assess the serotype of each isolate CST was used. Based on the
determinationofthepartialsequenceofthecapsularwzhgene,thismethodassignsacapsulartypeofanisolatewithina
singlePCRreactionusingmultipleprimersets.ThegeneticbackgroundofpneumococcalisolateswasassessedbyMLVA.
MLVAandCSTwereusedtocreateasnapshotoftheDutchpneumococcalpopulationcausinginvasivediseasebeforethe
introductionofthe7-valentpneumococcalconjugatevaccineintheNetherlandsin2006.Atotalof1154clinicalisolates
collectedandserotypedbytheNetherlandsReferenceLaboratoryforBacterialMeningitiswereincludedinthesnapshot.
The CST was successful in discriminating most serotypes present in our collection. MLVA demonstrated that isolates
belongingtosomeserotypeshadarelativelyhighgeneticdiversitywhilstotherserotypeshadaveryhomogeneousgenetic
background.MLVAandCSTappeartobevaluabletoolstodeterminethepopulationstructureofpneumococcalisolates
andareusefulinmonitoringtheeffectsofpneumococcalvaccination.
Citation:ElberseKEM,vandePolI,WitteveenS,vanderHeideHGJ,SchotCS,etal.(2011)PopulationStructureofInvasiveStreptococcuspneumoni
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