Pyrosequencing of Mytilus galloprovincialis cDNAs Tissue-Specific Expression Patterns 英文参考文献.docVIP

下载本文档

9
0
约4.44万字
约 10页
2017-05-12 发布于上海
举报
版权申诉

Pyrosequencing of Mytilus galloprovincialis cDNAs Tissue-Specific Expression Patterns 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Pyrosequencing of Mytilus galloprovincialis cDNAs Tissue-Specific Expression Patterns 英文参考文献

PyrosequencingofMytilusgalloprovincialiscDNAs: Tissue-SpecificExpressionPatterns JohnA.Craft1*,JackA.Gilbert2,BenTemperton2,KateE.Dempsey1¤a,KevinAshelford3,BelaTiwari4, TomH.Hutchinson2¤b,J.KevinChipman5 1BiologicalandBiomedicalSciences,GlasgowCaledonianUniversity,Glasgow,UnitedKingdom,2PlymouthMarineLaboratory,TheUniversityofLiverpool,Liverpool, United Kingdom, 3School of Biological Sciences, The University of Liverpool, Liverpool, United Kingdom, 4Natural Environment Research Council Environmental BioinformaticsCentre,CentreforEcologyandHydrology,Wallingford,UnitedKingdom,5SchoolofBiologicalSciences,UniversityofBirmingham,Birmingham,United Kingdom Abstract Background:Mytilusspeciesareimportantinmarineecologyandinenvironmentalqualityassessment,yettheirmolecular biology is poorly understood. Molecular aspects of their reproduction, hybridisation between species, mitochondrial inheritance,skewedsexratiosofoffspringandadaptationtoclimaticandpollutionfactorsarepriorityareas. Methodology/PrincipalFindings:Tostarttoaddressthissituation,expressedgenetictranscriptsfromM.galloprovincialis were pyrosequenced. Transcripts were isolated from the digestive gland, foot, gill and mantle of both male and female mussels.Intotal,175,547sequenceswereobtainedandforfootandmantle,90%ofthesequencescouldbeassembledinto contiguousfragmentsbutthisreducedto75%forthedigestiveglandandgill.Transcriptsrelatingtoproteinmetabolism andrespirationdominatedincludingribosomalproteins,cytochromeoxidasesandNADHdehydrogenasesubunits.Tissue specificvariationwasidentifiedintranscriptsassociatedwithmitochondrialenergymetabolism,withthedigestivegland and gill having the greatest transcript abundance. Using fragment recruitment it was also possible to identify sites of potentialsmallRNAsinvolvedinmitochondrialtranscriptionalregulation.SexratiosbasedonVitellineEnvelopReceptorfor LysinandVitellineCoatLysintranscriptabundances,indicatedthatanequalsexdistributionwasmaintained.Taxonomic profiling of the M. gall