Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS) 英文参考文献.docVIP

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Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS) 英文参考文献.doc

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Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS) 英文参考文献

ZhaoandLeungChineseMedicine2010,5:4 /content/5/1/4 RESEARCH OpenAccess QualityevaluationofmycelialAntrodia camphoratausinghigh-performanceliquid chromatography(HPLC)coupledwithdiodearray detectorandmassspectrometry(DAD-MS) SandyShuoZhao,KelvinSze-YinLeung* Abstract Background:Antrodiacamphorata(AC)isanimportantfungusnativetoTaiwaneseforestedregions.Scientific studieshavedemonstratedthatextractsofACpossessavarietyofpharmacologicalfunctions.Thisstudyaimsto identifythefullprofilefingerprintofnucleosidesandnucleobasesinmycelialACandtoassessthequalityoftwo commercialmycelialACproducts. Methods:High-performanceliquidchromatographycoupledwithdiodearraydetectorandmassspectrometry wasemployedtoidentifythemajorcomponentsinmycelialAC.Thechemicalseparationwascarriedoutusinga gradientprogramonareversephaseAlltimaC18 AQanalyticalcolumn(250×4.6mm,5μm)withthemobile phaseconsistingofdeionizedwaterandmethanol. Results:Tennucleosidesandnucleobases,twomaleimidederivatives,andasterolwereidentifiedasthemajor constituentsinmycelialAC.Thesegroupsofchemicalcompoundsconstitutethefirstchromatographicfingerprint asanindexforqualityassessmentofthismedicinalfungus. Conclusions:ThisstudyprovidesthefirstchromatographicfingerprinttoassessthequalityofmycelialAC. Background AfterthesuccessinmassproductionofACbyartifi- Antrodia camphorata (M. Zang C.H. Su) Sheng H. cial cultivation, a series of health supplements formu- Wu,RyvardenT.T.Chang(Polyporaceae)isaparasi- lated from AC has been launched with high market tic fungus on decayed wood or the inner wall of the value [3], and are increasingly popular in the Taiwan, heartwoodofCinnamomumkanehiraihay,atreeende- Japan, and other Asian regions. Counterfeit over-the- mic to Taiwan. Before Antrodia camphorata (AC) was counter AC products have been found and reported. first officially classified as aspecies in 1990, its medic- However,thereisnoreliablequalityassessmentmethod inal value had been greatly appreciated for many dec- toeval