Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects 英文参考文献.docVIP

Molecules 2011, 16, 9480-9494; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects Xian Qiong Liu 1,2, Hao Wu 1,*, Hong Li Yu 1, Teng Fei Zhao 1, Yao Zong Pan 1 and Run Jun Shi 1 1 Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210046, China 2 Hubei University of Chinese Medicine, Wuhan, 430061, China * Author to whom correspondence should be addressed; E-Mail: wuha; Tel.: +86-25 Fax: +86-25 Received: 24 October 2011; in revised form: 7 November 2011 / Accepted: 8 November 2011 / Published: 14 November 2011 Abstract: The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the nu