Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull 英文参考文献.docVIP

Molecules 2012, 17, 8079-8090; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull Aoxue Luo 1,?, Aoshuang Luo 2, Jiandong Huang 1 and Yijun Fan 1,?,* 1 Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China ? These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: yijunfan@. Received: 22 May 2012; in revised form: 20 June 2012/ Accepted: 22 June 2012 / Published: 5 July 2012 Abstract: A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant. Keywords: Boletus edulis Bull; polysaccharide; purification; antioxidant activity 1. Introduction Oxidative stress-induced cell damage triggers both the physiological process of aging and many pathological progressions that can eventually lead to serious health problems [1]. Antioxidants can reduce the cellular oxidative stress by inhibiting the formation of superoxide anions, and by detoxification of reactive oxygen species/reactive nitrogen species through upregulation of cellular defense mechanisms, such as superoxide dismutase