Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion System Is Organized in a Periodic Pattern of Foci 英文参考文献.docVIP

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Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion System Is Organized in a Periodic Pattern of Foci 英文参考文献.doc

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Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion System Is Organized in a Periodic Pattern of Foci 英文参考文献

QuantitativeImageAnalysisandModelingIndicatethe AgrobacteriumtumefaciensTypeIVSecretionSystemIs OrganizedinaPeriodicPatternofFoci ToddA.Cameron1,MarcusRoper2,PatriciaC.Zambryski1* 1DepartmentofPlantandMicrobialBiology,UniversityofCalifornia,Berkeley,California,UnitedStatesofAmerica,2DepartmentofMathematics,UniversityofCalifornia LosAngeles,LosAngeles,California,UnitedStatesofAmerica Abstract TheGramnegativeplantpathogenAgrobacteriumtumefaciensisuniquelycapableofgeneticallytransformingeukaryotic hostcellsduringtheinfectionprocess.DNAandproteinsubstratesaretransferredintoplantcellsviaatypeIVsecretion system(T4SS),whichformslargecell-envelopespanningcomplexesatmultiplesitesaroundthebacterialcircumference.To gainadetailedunderstandingofT4SSpositioning,thespatialdistributionoffluorescentlylabeledT4SScomponentswas quantitatively assessed to distinguish between random and structured localization processes. Through deconvolution microscopyfollowedbyFourieranalysisandmodeling,T4SSfociwerefoundtolocalizeinanon-randomperiodicpattern. These results indicate that T4SS complexes are dependent on an underlying scaffold or assembly process to obtain an organizeddistributionsuitableforeffectivedeliveryofsubstratesintohostcells. Citation: Cameron TA, Roper M, Zambryski PC (2012) Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion SystemIsOrganizedinaPeriodicPatternofFoci.PLoSONE7(7):e42219.doi:10.1371/journal.pone.0042219 Editor:IvoG.Boneca,InstitutPasteurParis,France ReceivedMarch31,2012;AcceptedJuly2,2012;PublishedJuly30,2012 Copyright: ? 2012 Cameron et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: TCand PZ weresupportedby NSF () grantMCB-0343566. MR was supportedby afellowship from the Miller Institute for Basic ResearchinSciences().Thefundershadno