Regulated Expressions of MMP-2, -9 by Diterpenoids from Euphorbia formosana Hayata 英文参考文献.docVIP

Molecules 2012, 17, 2082-2090; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Regulated Expressions of MMP-2, -9 by Diterpenoids from Euphorbia formosana Hayata Chia-Chun Yu 1,?, Ching-Ruey Hsieh 2,?, George Hsiao 3, Pi-Yu Chen 4, Meng-Lun Chang 1, Hwa-Wen Yin 5,Tzong-Huei Lee 1,* and Ching-Kuo Lee 4,* 1 Graduate Institute of Pharmacognosy, Taipei Medical University, 250 Wu Xin Street, Taipei 110, Taiwan; E-Mails: orchidbutterfly@ (C.-C.Y.); chang76819@.tw (M.-L.C.) 2 Taipei Medical University Hospital, Taipei Medical University, 252 Wu Xin Street, Taipei 110, Taiwan; E-Mail: tmc761038@.tw 3 Department of Pharmacology, Taipei Medical University, 250 Wu Xin Street, Taipei 110, Taiwan; E-Mail: geohsiao@.tw 4 School of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 110, Taiwan; E-Mail: e023089103@.tw 5 Taiwan Forestry Research Institute, No. 53, Nan-Hai Road, Taipei, 100, Taiwan; E-Mail: hwyin@.tw ? These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: thlee@.tw (T.-H.L.); cklee@.tw (C.-K.L.); Tel.: +886-2-2736-1661 ext. 6150; Fax: +886-2-2377-2265. Received: 29 November 2011; in revised form: 3 February 2012 / Accepted: 6 February 2012 / Published: 21 February 2012 Abstract: Two new abietane type diterpenoids, namely seco-helioscopinolide (1) and 3?,7?-dihydroxy-ent-abieta-8,13-diene-12,16-olide (2) were isolated from the aerial parts of Euphorbia formosana Hayata together with helioscopinolide A (3), helioscopinolide B (4), helioscopinolide C (5) and ent-(5?,8?,9?,10?,12?)-12-hydroxyatis-16-ene-3,14-dione (6). The structures of compounds 1?6 were elucidated by analyzing their spectroscopic data and comparison with the literature. Further biological tests by gelatin zymographic analysis revealed that 3?5 significantly up-regulated the expressions and activation of MMP-2 and -9 in human fibrosarcoma cell