RNA-Seq and find entering the RNA deep field 英文参考文献.docVIP

Roberts and Pachter Genome Medicine 2011,3:74 /content/3/11/74 RESEARCH HIGHLIGHT RNA-Seq and nd: entering the RNA deep eld Adam Roberts and Lior Pachter * 1 1,2 encountered by an astronomer attempting to detect a low magnitude star from images collected in a low resolution sky survey. e tradeo? between breadth and depth is one that astronomers have grappled with for a long time and have ultimately resolved with the development of telescopes that can limit the scope of a detector to areas of interest, along with guiding technology enabling deep sampling of a region over long exposures. is approach was the design principle for the Hubble Deep Field, which focused a powerful detector on the darkest portions of the sky. With the bright ‘foreground’ of nearby objects removed, an immense number of galaxies were discovered in what was previously thought to be empty space. Mercer et al. [5] have designed an analogous focused experiment for probing the transcriptome, RNA CaptureSeq, and describe a similar outcome: regions with only scattered coverage in genome-wide experi- ments are revealed to be loci with transcription of low- abundance RNAs. A key aspect of CaptureSeq is that the Abstract Initial high-throughput RNA sequencing (RNA-Seq) experiments have revealed a complex and dynamic transcriptome, but because it samples transcripts in proportion to their abundances, assessing the extent and nature of low-level transcription using this technique has been dicult. A new assay, RNA CaptureSeq, addresses this limitation of RNA-Seq by enriching for low-level transcripts with cDNA tiling arrays prior to high-throughput sequencing. This approach reveals a plethora of transcripts that have been previously dismissed as‘noise’, and hints at single- cell transcription ngerprints that may be crucial in dening cellular function in