Robust Food Anticipatory Activity in BMAL1-Deficient Mice 英文参考文献.docVIP

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Robust Food Anticipatory Activity in BMAL1-Deficient Mice 英文参考文献.doc

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Robust Food Anticipatory Activity in BMAL1-Deficient Mice 英文参考文献

RobustFoodAnticipatoryActivityinBMAL1-Deficient Mice JulieS.Pendergast1,WataruNakamura2,RioC.Friday1,FumiyukiHatanaka2,ToruTakumi2 ,Shin Yamazaki1* 1DepartmentofBiologicalSciences,VanderbiltUniversity,Nashville,Tennessee,UnitedStatesofAmerica,2OsakaBioscienceInstitute,Suita,Osaka,Japan Abstract Foodavailabilityisapotentenvironmentalcuethatdirectscircadianlocomotoractivityinrodents.Eventhoughnocturnal rodentsprefertoforageatnight,daytimefoodanticipatoryactivity(FAA)isobservedpriortoshortmealspresentedata scheduled time of day. Under this restricted feeding regimen, rodents exhibit two distinct bouts of activity, a nocturnal activityrhythmthatisentrainedtothelight-darkcycleandcontrolledbythemasterclockinthesuprachiasmaticnuclei (SCN)andadaytimeboutofactivitythatisphase-lockedtomealtime.FAAalsooccursduringfooddeprivation,suggesting that a food-entrainable oscillator (FEO) keeps time in the absence of scheduled feeding. Previous studies have demonstratedthattheFEOisanatomicallydistinctfromtheSCNandthatFAAisobservedinmicelackingsomecircadian genesessentialfortimekeepingintheSCN.Inthecurrentstudy,weoptimizedtheconditionsforexaminingFAAduring restrictedfeedingandfooddeprivationinmicelackingfunctionalBMAL1,whichiscriticalforcircadianrhythmgenerationin theSCN.WefoundthatBMAL1-deficientmicedisplayedFAAduringrestrictedfeedingin12hrlight:12hrdark(12L:12D)and 18L:6D lighting cycles, but distinct activity during food deprivation was observed only in 18L:6D. While BMAL1-deficient mice also exhibited robust FAA during restricted feeding in constant darkness, mice were hyperactive during food deprivationsoitwasnotclearthatFAAconsistentlyoccurredatthetimeofpreviouslyscheduledfoodavailability.Taken together, our findings suggest that optimization of experimental conditions such as photoperiod may be necessary to visualize FAA in genetically modified mice. Furthermore, the expression of FAA may be possible without a circadian oscillatorthatdependsonBMAL1. Citation:PendergastJS,Nakam