Role of the gp85Trans-Sialidases in Trypanosoma cruzi Tissue Tropism Preferential Binding of a Conserved Peptide Motif to the Vasculature In Vivo 英文参考文献.docVIP

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Role of the gp85Trans-Sialidases in Trypanosoma cruzi Tissue Tropism Preferential Binding of a Conserved Peptide Motif to the Vasculature In Vivo 英文参考文献.doc

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Role of the gp85Trans-Sialidases in Trypanosoma cruzi Tissue Tropism Preferential Binding of a Conserved Peptide Motif to the Vasculature In Vivo 英文参考文献

Roleofthegp85/Trans-SialidasesinTrypanosomacruzi TissueTropism:PreferentialBindingofaConserved PeptideMotiftotheVasculatureInVivo RenataR.Tonelli1,RicardoJ.Giordano2,ElenaMagdaBarbu3,AnaClaudiaTorrecilhas2,GersonS. Kobayashi2,RobertR.Langley4,WadihArap3,RenataPasqualini3,WalterColli2,MariaJu′liaM.Alves2* 1DepartamentodeMicrobiologia,ImunologiaeParasitologia,UniversidadeFederaldeSa?oPaulo,Sa?oPaulo,Brazil,2DepartamentodeBioqu?′mica,InstitutodeQu?′mica, UniversidadedeSa?o Paulo,Sa?o Paulo,Brazil,3DavidH.KochCenter,TheUniversityofTexasMDAndersonCancerCenter,Houston,Texas,UnitedStatesofAmerica, 4DepartmentofCancerBiology,TheUniversityofTexasMDAndersonCancerCenter,Houston,Texas,UnitedStatesofAmerica Abstract Background: Transmitted by blood-sucking insects, the unicellular parasite Trypanosoma cruzi is the causative agent of Chagas’ disease, a malady manifested in a variety of symptoms from heart disease to digestive and urinary tract dysfunctions.Thereasonsforsuchorganpreferencehavebeenamatterofgreatinterestinthefield,particularlybecause the parasite can invade nearly every cell line and it can be found in most tissues following an infection. Among the molecularfactorsthatcontributetovirulenceisalargemultigenefamilyofproteinsknownasgp85/trans-sialidase,which participatesincellattachmentandinvasion.Butwhethertheseproteinsalsocontributetotissuehominghadnotyetbeen investigated. Here, a combination of endothelial cell immortalization and phage display techniques has been used to investigatetheroleofgp85/trans-sialidaseinbindingtothevasculature. Methods: Bacteriophage expressing an important peptide motif (denominated FLY) common to all gp85/trans-sialidase proteinswasusedasasurrogatetoinvestigatetheinteractionofthismotifwiththeendotheliumcompartment.Forthat purpose phage particles were incubated with endothelial cells obtained from different organs or injected into mice intravenously and the number of phage particles bound to cells or tissues was determined. Binding of p